Abstract
Aerobic bacterial strains from the salt water of Lake Red (Sovata, Romania) were cultivated. More than half of the 80 strains were G− and formed motile straight rods. Only a few strains produced acid from d-glucose and reduced nitrate to nitrite. Optimum NaCl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. On the basis of the 16S rRNA gene sequence similarity almost half of the strains were identified as members of genus Halomonas. Other strains belonged to genera Marinobacter, Psychrobacter, Serratia, Morganella (γ-Proteobacteria), Bacillus, Exiguobacterium, Planococcus (Firmicutes), and Arthrobacter, Micrococcus, Microbacterium, and Nesterenkonia (Actinobacteria).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ARDRA:
-
amplified rDNA restriction analysis
- CFU:
-
colony forming units
- MOA:
-
modified oligotrophic agar
- OA:
-
oligotrophic agar
- PCR:
-
polymerase chain reaction
- SWA:
-
seawater agar
References
Alinei R., Ionica A., Cheratoiu C., Cicu A., Gheorghita D., Guzu D., Falamas R., Bucurenciu C., Arimie B., Mironescu M., Oprean L.: Isolation of halophilic microorganisms in the saline lakes from Ocna Sibiului and analysis of red pigments production. Acta Univ.Cibiniensis Ser. E Food Technol.10, 21–33 (2006).
Anan’ina L.N., Plotnikova E.G., Gavrish E.Y., Demakov V.A., Evtushenko L.I.: Salinicola socius gen.nov., sp.nov., a moderately halophilic bacterium from a naphthalene-utilizing microbial association. Microbiology76, 324–330 (2007).
Arahal D.R., Ludwig W., Schleifer K.H., Ventosa A.: Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Internat.J.Syst.Evol.Microbiol.52, 241–249 (2002).
Arahal D.R., Vreeland R.H., Litchfield C.D., Mormile M.R., Tindall B.J., Oren A., Bejar V., Quesada E., Ventosa A.: Recommended minimal standards for describing new taxa of the family Halomonadaceae. Internat.J.Syst.Evol.Microbiol. 57, 2436–2446 (2007).
Barrow G.I., Feltham R.K.A.: Cowan and Steel’s Manual for the Identification of Medical Bacteria. Cambridge University Press, Cambridge (UK) 2003.
Darie N., Oprean L., Ognean F.C., Ognean M.: Testing of some enzymatic levels in Ocna Sibiului lakes therapeutic mud.Acta Univ. Cibiniensis Ser. F Chemia9, 21–28 (2006).
Demergasso C., Casamayor E.O., Chong G., Galleguillos P., Escudero L., Pedros-Alio C.: Distribution of prokaryotic genetic diversity in athalassohaline lakes of the Atacama Desert, Northern Chile. FEMS Microbiol.Ecol.48, 57–69 (2004).
Demergasso C., Escudero L., Casamayor E.O., Chong G., Balague V., Pedros-Alio C.: Novelty and spatio-temporal heterogeneity in the bacterial diversity of hypersaline Lake Tebenquiche (Salar de Atacama). Extremophiles12, 491–504 (2008).
Dong H., Zhang G., Jiang H., Yu B., Chapman L.R., Lucas C.R., Fields M.W.: Microbial diversity in sediments of saline Qinghai Lake, China: linking geochemical controls to microbial ecology. Microb.Ecol.51, 65–82 (2006).
Glatz R.E., Lepp P.W., Ward B.B., Francis C.A.: Planktonic microbial community composition across steep physical/chemical gradients in permanently ice-covered Lake Bonney, Antarctica. Geobiology4, 53–67 (2006).
Kim K.K., Jin L., Yang H.C., Lee S.T.: Halomonas gomseomensis sp.nov., Halomonas janggokensis sp.nov., Halomonas salaria sp.nov. and Halomonas denitrificans sp.nov., moderately halophilic bacteria isolated from saline water. Internat.J.Syst.Evol. Microbiol.57, 675–681 (2007).
Lee J.C., Jeon C.O., Lim J.M., Lee S.M., Lee J.M., Song S.M., Park D.J., Li W.J., Kim C.J.: Halomonas taeanensis sp.nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Internat.J.Syst.Evol.Microbiol.55, 2027–2032 (2005).
Li W.J., Chen H.H., Kim C.J., Zhang Y.Q., Park D.J., Lee J.C., Xu L.H., Jiang C.L.: Nesterenkonia sandarakina sp.nov. and Nesterenkonia lutea sp.nov., novel actinobacteria, and emended description of the genus Nesterenkonia. Internat.J.Syst.Evol. Microbiol.55, 463–466 (2005).
Mutlu M.B., Martínez-García M., Santos F., Peña A., Guven K., Anton J.: Prokaryotic diversity in Tuz Lake, a hypersaline environment in Inland Turkey. FEMS Microbiol.Ecol.65, 474–483 (2008).
Navarro J.B., Moser D.P., Flores A., Ross C., Rosen M.R., Dong H., Zhang G., Hedlund B.P.: Bacterial succession within an ephemeral hypereutrophic Mojave Desert playa lake. Microb.Ecol.57, 307–320 (2009).
Poli A., Esposito E., Orlando P., Lama L., Giordano A., de Appolonia F., Nicolaus B., Gambacorta A.: Halomonas alkaliantarctica sp.nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium. Syst.Appl.Microbiol.30, 31–38 (2007).
Shivaji S., Gupta P., Chaturvedi P., Suresh K., Delille D.: Marinobacter maritimus sp.nov., a psychrotolerant strain isolated from sea water of the subantarctic Kerguelen islands. Internat.J.Syst.Evol.Microbiol.55, 1453–1456 (2005).
Smibert R.M., Krieg N.R.: Phenotypic characterization, in P. Gerhardt, R.G.E. Murray, W.A. Wood, N.R. Krieg (Eds): Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington (DC) 1994.
Ventosa A., Nieto J.J., Oren A.: Biology of moderately halophilic aerobic bacteria. Microbiol.Mol.Biol.Rev.62, 504–544 (1998).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Borsodi, A.K., Kiss, R.I., Cech, G. et al. Diversity and activity of cultivable aerobic planktonic bacteria of a saline Lake located in Sovata, Romania. Folia Microbiol 55, 461–466 (2010). https://doi.org/10.1007/s12223-010-0077-7
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12223-010-0077-7