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Hematopoietic- and Neurologic-Expressed Sequence 1 Expression in the Murine GL261 and High-Grade Human Gliomas

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Pathology & Oncology Research

Abstract

The hematopoietic- and neurologic-expressed sequence 1 (Hn1) gene encodes a highly conserved protein that is expressed in developing and regenerating tissues. In this study, Hn1 expression was evaluated in human and murine malignant gliomas. Hn1 mRNA and protein were detected in the murine GL261 glioma cell line and in GL261 brain tumors in vivo. HN1 is also expressed in human U118MG and U87MG cell lines. Evaluation of human brain tumors using an anti-Hn1 polyclonal antibody detected strong immunoreactivity in high-grade (WHO III and IV) malignant gliomas. The rate of GL261 cell proliferation in vitro was unaltered by Hn1 depletion using an anti-Hn1 siRNA. However, tumors established from Hn1-depleted GL261 cells formed significantly smaller volumes than those established from control-treated cells. These data suggest a role for Hn1 in the biology of malignant brain tumors.

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Acknowledgements

Financial support for these studies was provided by the Evelyn F. and William L. McKnight Brain Institute of the University of Florida and the National Institutes of Health (AI058256) to J.K.H. We are thankful to Ms. Irene Zolotukhin for manufacturing the adeno-associated viruses utilized in this study. The anti-Hn1 antibody used in this study was generated at EnCor Biotechnology, Inc., Gainesville, Florida. A portion of these data were presented at the 2007 annual meetings of the Society for Neuroscience in San Diego, CA and Society for Neuro-oncology in Dallas, TX.

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Authors and Affiliations

  1. Department of Pharmacology & Therapeutics, University of Florida College of Medicine, P.O. Box 100267, 1600 SW Archer Rd, Gainesville, FL, 32610-0267, USA

    Katharine M. Laughlin, Defang Luo, Che Liu & Jeffrey K. Harrison

  2. Department of Neuroscience, University of Florida College of Medicine, Gainesville, FL, 32610-0267, USA

    Gerry Shaw

  3. Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL, 32610-0267, USA

    Kenneth H. Warrington Jr.

  4. Department of Pathology, University of Florida College of Medicine, Gainesville, FL, 32610-0267, USA

    Jingxin Qiu & Anthony T. Yachnis

Authors
  1. Katharine M. Laughlin
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  2. Defang Luo
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  3. Che Liu
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  4. Gerry Shaw
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  5. Kenneth H. Warrington Jr.
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  6. Jingxin Qiu
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  7. Anthony T. Yachnis
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  8. Jeffrey K. Harrison
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Correspondence to Jeffrey K. Harrison.

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Laughlin, K.M., Luo, D., Liu, C. et al. Hematopoietic- and Neurologic-Expressed Sequence 1 Expression in the Murine GL261 and High-Grade Human Gliomas. Pathol. Oncol. Res. 15, 437–444 (2009). https://doi.org/10.1007/s12253-008-9147-4

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  • DOI: https://doi.org/10.1007/s12253-008-9147-4

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