Abstract
The inhalation exposure of adult male rats to 7.9 μmol/l (200 ppm) of trichloroethylene for 4 days 6 h each day led to an accumulation of the solvent in the perirenal fat 17 h after the last exposure. Exposures to 20 μmol/l (500 ppm) of 1,1,1-trichloroethane caused similar accumulation. The latter rats presented no changes in their behaviour in an open-field test whereas the motor behaviour of the animals exposed to trichloroethylene was more active in comparison to controls 1 h after the exposure on the 4th day. Trace effects of trichloroethylene on emotional behaviour of the same rats could be seen 17 h after the last exposure.
Further exposures on the 5th day increased brain, liver, lung and blood contents of trichloroethylene and 1,1,1-trichloroethane. A slight decrease in brain RNA content was found in the 1,1,1-trichloroethane exposed rats while RNA decreased significantly after the fifth day of trichloroethylene inhalation. The exposure to 1,1,1-trichloroethane on the 5th day depressed also the microsomal cytochrome P-450 content in liver of rats whereas trichloroethylene increased the hemochrome content slightly at the same time.
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References
Åstrand, I: Uptake of solvents in the blood and tissues of man. Scand. J. Work Environm. Hlth 1, 199–218 (1975)
Bättig, K., Grandjean, E.: Chronic effects of trichlorethylene on rat behaviour. Arch. environm. Hlth 7, 694–699 (1963)
Bonse, G., Henschler, D.: Chemical reactivity, biotransformation, and toxicity of polychlorinated aliphatic compounds. CRC crit. Rev. Toxicol. 10, 395–409 (1976)
Chalupa, B., Synková, J., Sevcik, M.: The assessment of electroencephalographic changes and memory disturbances in acute intoxications with industrial poisons. Brit. J. industr. Med. 17, 238–241 (1960)
Conney, A. H.: Pharmacological implications of microsomal enzyme induction. Pharmacol. Rev. 19, 317–366 (1967)
Eriksson, K., Wallgren, H.: Behaviour of rats under the influence of ethyl alcohol in an open-field situation. Scand. J. Psychol. 8, 257–267 (1067)
Fleming, E. W., Tewari, S., Noble, E. P.: Effects of chronic ethanol ingestion on brain aminoacyl-tRNA synthetases and tRNA. J. Neurochem. 24, 553–560 (1975)
Grandjean, E.: Trichlorethylene effects on animal behaviour. Arch. environm. Hlth 1, 106–108 (1960)
Grandjean, E., Münchinger, R., Trurian, V., Haas, P. A., Knoepfel, H. K., Rosenmund, H.: Investigation into the effects of exposure to trichlorethylene in mechanical engineering. Brit. J. industr. Med. 12, 131–142 (1955)
Henschler, D., Bonse, G., Greim, H.: Carcinogenic potential of chlorinated ethylenes: Tentative molecular rules. INSERM Symposia Series 52, 171–175 (1976)
Herd, P., Martin, H. F.: Effect of 1,1,1-trichloroethane on mitochondrial metabolism. Biochem. Pharmacol. 24, 1179–1185 (1975)
Hissin, P. J., Hilf, R.: A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analyt. Biochem. 74, 214–226 (1976)
Ikeda, M., Ohtsuji, H.: A comparative study of the excretion of Fujiwara reaction-positive substances in urine of humans and rodents given trichloro- or tetrachloroderivatives of ethane and ethylene. Brit. J. industr. Med. 29, 99–104 (1972)
Konietzko, H., Elster, J., Bencsàth, A., Drysch, H., Weichardt, H.: Psychomotorische Reaktionen unter definierter Trichloräthylenbelastung. Arch. Toxicol. 33, 129–139 (1975)
Lagerspetz, K. M. J.: Studies on the aggressive behaviour of mice. Ann. Acad. Sci. Fenn. Ser. B 131, 1–131 (1964)
Lowry, O. H., Rosebrough, H. J., Farr, A. L., Randall, R. J.: Protein measurement with Folin reagent. J. biol. Chem. 193, 265–275 (1951)
Marks, N., Stern, F., Lajtha, A.: Changes in proteolytic enzymes and proteins during maturation of the brain. Brain Res. 86, 307–322 (1975)
Morimoto, H., Ferchmin, P. A., Bennett, E. L.: Spectrophotometric analysis of RNA and DNA using cetyltrimethylammonium bromide. Analyt. Biochem. 62, 436–448 (1974)
Mørland, J., Sjetnan, A. E.: Reduced incorporation of (3H) leucine into cerebral proteins after longterm ethanol treatment. Biochem. Pharmacol. 25, 220–221 (1976)
Norpoth, K., Witting, U., Springorum, M.: Induction of microsomal enzymes in rat liver by inhalation of hydrocarbon solvents. Int. Arch. Arbeitsmed. 33, 315–321 (1974)
Orrenius, S., Ernster, L.: Microsomal cytochrome P-450 linked monooxygenase systems in mammalian tissues. In: Molecular mechanisms of oxygen activation (O. Hayaishi, Ed.), pp. 215–244. New York-London: Academic Press 1975
Pelkonen, O., Vainio, H.: Spectral interactions of a series of chlorinated hydrocarbons with cytochrome P-450 of liver microsomes from variously-treated rats. FEBS Letters 51, 11–14 (1975)
Renis, M., Giovine, A., Bertolino, A.: Protein synthesis in mitochondrial and microsomal fractions from rat brain and liver after acute or chronic ethanol administration. Life Sci. 16, 1447–1458 (1975)
Rowe, V. K., Wujkowski, T., Wolf, M. A., Sadek, S. E., Stewart, R. D.: Toxicity of a solvent mixture of 1,1,1-trichloroethane and tetrachloroethylene as determined by experiments on laboratory animals and human subjects. Amer. industr. Hyg. Ass. J. 24, 541–554 (1963)
Smith, G. F.: Trichlorethylene: A review. Brit. J. industr. Med. 23, 249–262 (1966)
Stewart, R. D.: Trichlorethanes. In: Encyclopaedia of occupational health of safety, Vol. II, pp. 1432–1433. Geneva: I.L.O. 1972
Stewart, R. D., Dodd, H. C., Gay, H. H., Erley, D. S.: Experimental human exposure to trichloroethylene. Arch. environm. Hlth 20, 64–71 (1970)
Tabershaw, I. R.: Trichlorethylene. In: Encyclopaedia of occupational health and safety, Vol. II, pp. 1433–1434. Geneva: I.L.O. 1972
Vainio, H., Aitio, A.: Enhancement of microsomal drug hydroxylation and glucuronidation in rat liver by phenobarbital and 3-methylcholantrene in combination. Acta pharmacol. (Kbh.) 34, 130–140 (1974)
Vainio, H., Parkki, M. G., Marniemi, J.: Effects of aliphatic chlorohydrocarbons on drug-metabolizing enzymes in rat liver in vitro. Xenobiotica 6, 599–604 (1976)
Van Duuren, B. L., Banerjee, S.: Covalent interaction of metabolites of carcinogen trichloroethylene in rat hepatic microsomes. Cancer Res. 36, 2419–2422 (1976)
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Savolainen, H., Pfäffli, P., Tengén, M. et al. Trichloroethylene and 1,1,1-trichloroethane: effects on brain and liver after five days intermittent inhalation. Arch. Toxicol. 38, 229–237 (1977). https://doi.org/10.1007/BF00293657
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DOI: https://doi.org/10.1007/BF00293657