Abstract
Pure trichloroethylene (tri), stabilized by an amine base, was administered by inhalation at 0, 100, and 500 ppm for 6 h/day, 5 days/week, for 18 months to mice, rats and Syrian hamsters of both sexes. No significant increase in tumor formation was observed in any species or dosing group, except in malignant lymphomas, which were increased in female mice in the following incidence rates: 9/29 (controls), 17/30 (100 ppm), and 18/28 (500 ppm). Whether or not this high occurrence of lymphomas, which is peculiar to this strain of mice (NMRI) has any relationship to tri-exposure, cannot be decided upon by the present experiment. It is concluded that from these findings no indication for a carcinogenic potential of pure trichloroethylene can be deduced.
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References
Cochran, W. C.: Some methods for strengthening the common Chi-square tests. Biometrics 10, 417–454 (1954)
Cole, R. K., Furth, M. D.: Experimental studies on the genetics of spontaneous leukemia in mice. Cancer Res. 1, 957–965 (1941)
Cox, D. R.: Regression models and life-tables (with discussion). J. Roy. Statist. Soc. B 34, 184–220 (1972)
Deerberg, F., Müller-Peddinghaus, R.: Vergleichende Untersuchungen über die Häufigkeit von Spontanerkrankungen und Spontantodesfällen bei weibl. konventionellen und bei spezifiziert pathogenfreien NMRI/HAN-Mäusen. Z. Versuchstierkd. 12, 341–347 (1970)
Deerberg, F., Pittermann, W., Rapp, K.: Der Lungentumor der Maus: ein progressiver Tumor. Vet. Pathol. 11, 439–441 (1974)
Gardener, M. B.: Unusually high incidence of spontaneous lymphomas in wild house mice. J. Natl. Cancer Inst. 50, 1571–1579 (1973)
Grasso, P., Crampton, R. F., Hooson, J.: The mouse and carcinogenicity testing. BIBRA-paper, Carshalton, Surrey, England, July 1977
Greim, H., Bonse, G., Radwan, Z., Reichert, D., Henschler, D.: Mutagenicity in vitro and potential carcinogenicity of chlorinated ethylenes as a function of metabolic oxirane formation. Biochem. Pharmacol. 24, 2013–2017 (1975)
Gross, L.: “Spontaneous” leukemia developing in C3H mice following inoculation in infancy, with AK-leukemic extracts, or AK-embryos. Proc. Soc. Exp. Biol. Med. 76, 27–36 (1951)
Henschler, D., Bonse, G.: Metabolic activation of chlorinated ethylene derivatives. Adv. Pharmacol. Ther. 9, 123–130 (1978)
Henschler, D., Eder, E., Neudecker, T., Metzler, M.: Carcinogenicity of trichloroethylene; fact or artifact? Arch. Toxicol. 37, 233–236 (1977)
Henschler, D., Hoos, W. R., Fetz, H., Dallmeier, E., Metzler, M.: Reactions of trichloroethylene epoxide in aqueous systems. Biochem. Pharmacol. 28, 543–548 (1979)
Ito, T.: Pathogenesis of thymic lymphoma induced by urethan in mice. Z. Krebsforsch. 66, 267–273 (1964)
Kaplan, E. L., Meier, P.: Nonparametric estimation from incomplete observations. J. Am. Statist. Ass. 53, 457–481 (1958)
Kirschbaum, A., Strong, L. C., Gardner, W. U.: Influence of methylcholanthrene on age incidence of leukemia in several strains of mice. Proc. Soc. Exp. Med. 45, 287–289 (1940)
Krüger, G. R. F.: Chronic immunosuppression and lymphomagenesis in man and mice. Natl. Cancer Inst. Monogr. 35, 183–190 (1972)
Krüger, G. R. F., Konorza, G.: Classification of animal lymphomas. Exp. Hematol. 7, 305–314, (1979)
Law, L. W.: The induction of leukemia in mice following percutaneous application of 9,10-dimethyl-1,2- benzanthracene. Cancer Res. 1, 564–571 (1941)
Liebermann, M., Kaplan, H. S.: Leukemogenic activity of filtrates from radiation-induced lymphoid tumors of mice. Science 130, 387–388 (1959)
Loprieno, N., Barale, R., Rossi, A. M.: In vivo mutagenicity studies with trichloroethylene and other solvents. Mutat. Res. (In press, 1979)
Luz, A.: The range of incidence of spontaneous neoplastic and non neoplastic lesions of the laboratory mouse. Z. Versuchstierkd. 19, 342–343 (1977)
Mantel, N.: Chi-square tests with one degree of freedom: extensions of the Mantel-Haenzel procedure. J. Am. Statist. Ass. 58, 690–700 (1963)
Mantel, N.: Evaluation of survival data and two new rank order statistics arising in its consideration. Cancer Chemother. Rep. 50, 163–170 (1966)
McClure, H., Chapman, W. L., Hooper, Jr., B. E., Smith, F. G.: The digestive system. In: Pathology of laboratory animals, vol. I. Benirschke, K. (ed.), pp. 175–317. Berlin, Heidelberg, New York: Springer 1978
Mider, G. B., Morton, J. J.: The effect of methylcholanthrene on the latent period of lymphomatosis in dilute brown mice. Am. J. Cancer 37, 355–363 (1939)
Miller, J. F. A. P.: Etiology and pathogenesis of mouse leukemia. Adv. Cancer Res. 6, 291–368 (1961)
Müller-Peddinghaus, R.: Die murine Leukose, Inaugural Dissertation, Tierärztl. Hochschule, Hannover 1972
Murphy, E. D.: Characteristic tumors. In: Biology of the laboratory mouse, 2nd ed. Green, L.E. (ed.), pp. 521–562. New York, Toronto, Sydney, London: McGraw Hill Book Company 1966
Natl. Cancer Inst.: Carcinogenesis bioassay of trichloroethylene. Carcinogenesis Tech. Rep. Ser. No. 2, DHEW Publ. No. (NIH) 76-802, Washington, D.C., 1976
Neø, B. A., Ulrich, K.: Activation of C-type virus during chemically induced leukemogenesis in mice. Cancer Res. 38, 725–735 (1978)
Saffiotti, U., Montesano, R., Sellakumar, A. R., Cefis, F., Kaufman, D. G.: Respiratory tract carcinogenesis in hamsters induced by different numbers of administrations of benzo(a)pyrene and ferric-oxide. Cancer Res. 32, 1073–1081 (1972)
Shahin, M. M., von Borstel, R. C.: Mutagenic and lethal effects of α-benzenehexachloride, dibutylphthalate and trichloroethylene in Saccharomyces Cerevisiae. Mutat. Res. 48, 173–180 (1977)
Toth, B., Shubik, Ph.: Studies with malignant lymphomas: possible interaction problems between chemical and viral inducing agents. Natl. Cancer Inst. Monogr. 22, 313–328 (1966)
Toth, B., Della Porta, G., Shubik, P.: The occurrence in malignant lymphomas in urethan treated Swiss-mice. Br. J. Cancer 15, 322–326 (1961)
Van Duuren, B. L.: On the possible mechanism of carcinogenic action of vinyl chloride. Ann. N.Y. Acad. Sci. 246, 258–267 (1975)
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Henschler, D., Romen, W., Elsässer, H.M. et al. Carcinogenicity study of trichloroethylene by longterm inhalation in three animal species. Arch. Toxicol. 43, 237–248 (1980). https://doi.org/10.1007/BF00366179
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DOI: https://doi.org/10.1007/BF00366179