Summary
An ultrastructural study of endocrine cells was performed in the pancreas of rats treated with a single dose of streptozotocin on the day of birth. Most of the B cells present at birth were destroyed by streptozotocin but some survived and could again synthesize insulin after eliminating their altered fragments in phago-lysosome structures. New B cells were produced primarily by the formation of new islets from the small pancreatic ducts. A study of mitosis in colchicine treated animals showed that most B cells present on day 4 were formed by mitosis of undifferentiated cells. No significant division of preexisting differentiated B cells could be shown in streptozotocin treated rats.
B cell regeneration in this newborn rat model is thus explained primarily by budding of new islets from the ducts.
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Arison RN, Ciaccio EI, Glitzer MS, Cassaro JA, Pruss BS (1967) Light and electron microscopy of lesions in rats rendered diabetic with streptozotocin. Diabetes 16:51–56
Cantenys D, Portha B, Dutrillaux MC, Hollande E, Roze C, Picon L (1981) Histogenesis of the endocrine pancreas in newborn rats after destruction by streptozotocin. An immunocytochemical study. Virchows Archiv [Cell Pathol] 35:109–122
Howell SL, Whitfield M (1972) Ultrastructural studies of the destruction of guinea-pig pancreatic B cells after injection of streptozotocin. Horm Metab Res 4:349–354
Johnson DD (1950) Alloxan administration in the guinea-pig. A study of the regenerative phase in the islands of Langerhans. Endocrinology 47:393–398
Lazarow A (1952) Spontaneous recovery from alloxan diabetes in the rat. Diabetes 1:363–370
Lazarus SS, Shapiro SH (1972) Serial morphologic changes in rabbit pancreatic islet cells after streptozotocin. Lab Invest 27:174–183
Lazarus SS, Shapiro SH (1973) Comparison of morphologic changes in nuclei of rabbit pancreatic islet B-cells induced by streptozotocin, alloxan and in vitro necrosis. Lab Invest 29:90–98
Like AA, Appel MC, Williams RM, Rossini AA (1978) Streptozotocin-induced pancreatic insulitis in mice. Morphologic and physiologic studies. Lab Invest 38:470–486
Orci L, Amherdt M, Malaisse-Lagae F, Ravazzola M, Malaisse WJ, Perrelet A, Renold AE (1976) Islet cell membrane alteration by diabetogenic drugs. Lab Invest 34:451–454
Portha B (1980) Le diabète expérimental chez le nouveau-né de rat et son évolution à long terme. Thèse Doct Sc Nat, Paris, 101 p
Portha B, Levacher C, Picon L, Rosselin G (1974) Diabetogenic effect of streptozotocin in the rat during the perinatal period. Diabetes 23:889–895
Portha B, Picon L, Rosselin G (1979) Chemical diabetes in the adult rat as the spontaneous evolution of neonatal diabetes. Diabetologia 17:371–377
Shinkawa Y, Kataoka K, Fujita H (1973) Electron microscopic observations on the pancreatic B cell in streptozotocin-induced hyperglycémic rats. Hiroshima J Med Sci 22:447–459
Steiner H, Oelz O, Zahn G, Froesch ER (1970) Studies on islet regeneration, hyperplasia and intrainsular interrelations in long-lasting streptozotocin diabetes in rats. Diabetologia 6:558–564
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M.C.D. is Assistant and C.R. is Maître Assistant at the P and M Curie University. This work was supported in part by ATP Inserm 79–106
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Dutrillaux, M.C., Portha, B., Rozé, C. et al. Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin. Virchows Archiv B Cell Pathol 39, 173–185 (1982). https://doi.org/10.1007/BF02892846
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DOI: https://doi.org/10.1007/BF02892846