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In vivo visualization of nitric oxide and interactions among platelets, leukocytes, and endothelium following hemorrhagic shock and reperfusion

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Abstract

Objectives

We examined changes in nitric oxide (NO) distribution in the mesenteric microcirculation after hemorrhagic shock and reperfusion (H/R), and correlated NO production to leukocyte and platelet behavior.

Materials and methods

The behavior of leukocytes and platelets in mesenteric venules was observed by intravital microscopy at 0.5 and 24 h after H/R in male Wistar rats. Transvascular leakage of fluorescein isothiocyanate-labeled albumin was assessed by epi-illumination. The NO-sensitive dye, 4,5-diaminofluorescein diacetate, was used for imaging NO release.

Results

H/R significantly increased vascular albumin leakage and adhesion of leukocytes and platelets (P < 0.05). In H/R 0.5 h rats, NO production in the venular endothelium declined. However, NO production was elevated in H/R 24 h rats in mast cells (P < 0.05). Leukocyte adherence, platelet adherence, and venular permeability were attenuated by iNOS inhibition.

Conclusion

Mesenteric endothelial cell dysfunction after H/R 0.5 h is associated with reduced NO, whereas after H/R 24 h is related to increase NO in mast cells.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Ehime University School of Medicine, Shitsukawa, Toon, Ehime, 791-0295, Japan

    Mie Hiratsuka, Tomihiro Katayama, Kazuhiko Uematsu & Masaharu Ito

  2. Department of Obstetrics and Gynecology, Uwajima City Hospital, Uwajima, Ehime, 798-8510, Japan

    Masaki Kiyomura

Authors
  1. Mie Hiratsuka
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  2. Tomihiro Katayama
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  3. Kazuhiko Uematsu
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  4. Masaki Kiyomura
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  5. Masaharu Ito
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Correspondence to Tomihiro Katayama.

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Responsible Editor: M. Katori.

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Hiratsuka, M., Katayama, T., Uematsu, K. et al. In vivo visualization of nitric oxide and interactions among platelets, leukocytes, and endothelium following hemorrhagic shock and reperfusion. Inflamm. Res. 58, 463–471 (2009). https://doi.org/10.1007/s00011-009-0011-0

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  • DOI: https://doi.org/10.1007/s00011-009-0011-0

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