Skip to main content
Log in

Leukocyte 8-hydroxydeoxyguanosine and aromatic DNA adduct in coke-oven workers with polycyclic aromatic hydrocarbon exposure

  • Original Article
  • Published:
International Archives of Occupational and Environmental Health Aims and scope Submit manuscript

Abstract

Objective

To investigate the potential for exposure to polycyclic aromatic hydrocarbons (PAHs) to induce oxidative DNA damage, we conducted a cross-sectional study in coke-oven workers employed at an iron–steel factory.

Methods

The study population contained 119 coke-oven workers from different work areas of the oven and 38 controls. Personal information on age, employment duration, smoking habit and alcohol consumption was obtained at an interview. Leukocyte 8-hydroxydeoxyguanosine (8-OHdG) was measured by high performance liquid chromatography with electrochemical detection. Leukocyte aromatic DNA adducts as effective dose, and urinary 1-hydroxypyren as internal dose, were also measured, and used to analyze the relationship of 8-OHdG with other biomarkers for PAH exposure, tobacco smoke and alcohol consumption.

Results

The leukocyte 8-OHdG revealed a wide inter-individual variation. The highest 8-OHdG level was detected in bottom-workers of the coke-oven. There were significant differences among the four different work areas (P=0.02). We could not find significant correlation between 8-OHdG levels and urinary 1-hydroxypyrene, but a weakly positive correlation was found between 8-OHdG and leukocyte aromatic DNA adducts among all subjects (r=0.19 P=0.03). We could not observe any effect of smoking and alcohol drinking on 8-OHdG production.

Conclusion

We could not find clear evidence that PAH exposure induces oxidative DNA damage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

References

  • Autrup H, Daneshvar B, Dragsted LO, Gamborg M, Hansen AM, Loft S, Okkels H, Nielsen F, Nielsen PS, Raffn E, Wallin H, Knudsen LE (1999) Biomarkers for exposure to ambient air pollution—comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress. Environ Health Perspect 107:233–238

    CAS  Google Scholar 

  • Beach AC, Gupta RC (1992) Human biomonitoring and the 32P-postlabeling assay. Carcinogenesis 13:1053–1074

    CAS  PubMed  Google Scholar 

  • Bessho T, Tano K, Kasai H, Ohtsuka E, Nishimura S (1993) Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in human cells. J Biol Chem 268:19416–19421

    CAS  PubMed  Google Scholar 

  • Bianchini F, Jaeckel A, Vineis P, Martinez-Garcia C, Elmstahl S, van Kappel AL, Boeing H, Ohshima H, Riboli E, Kaaks R (2001) Inverse correlation between alcohol consumption and lymphocyte levels of 8-hydroxydeoxyguanosine in humans. Carcinogenesis 22:885–890

    Article  CAS  PubMed  Google Scholar 

  • Bouchard M, Viau C (1999) Urinary 1-hydroxypyrene as a biomarker of exposure to polycyclic aromatic hydrocarbons: biological monitoring strategies and methodology for determining biological exposure indices for various work environments. Biomarkers 4:159–187

    Article  CAS  Google Scholar 

  • Carstensen U, Hou SM, Alexandrie AK, Hogstedt B, Tagesson C, Warholm M, Rannug A, Lambert B, Axmon A, Hagmar L (1999) Influence of genetic polymorphisms of biotransformation enzymes on gene mutations strand breaks of deoxyribonucleic acid and micronuclei in mononuclear blood cells and urinary 8-hydroxydeoxyguanosine in potroom workers exposed to polyaromatic hydrocarbons. Scand J Work Environ Health 25:351–360

    CAS  PubMed  Google Scholar 

  • Cavalieri EL, Rogan EG (1995) Central role of radical cations in metabolic activation of polycyclic aromatic hydrocarbons. Xenobiotica 25:677–688

    CAS  PubMed  Google Scholar 

  • Cheng KC, Cahill DS, Kasai H, Nishimura S, Loeb LA (1992) 8-Hydroxyguanine, an abundant form of oxidative DNA damage causes G→T and A→C substitutions. J Biol Chem 267:166–172

    CAS  PubMed  Google Scholar 

  • Farmer FB (1995) Monitoring of human exposure to carcinogens through DNA and protein adduct determination. Toxicol Lett 82–83:757–762

    Google Scholar 

  • Floyd RA (1990) The role of 8-hydroxyguanine in carcinogenesis. Carcinogenesis 11:1447–1450

    CAS  PubMed  Google Scholar 

  • Hara K, Hanaoka T, Yamano Y, Itani T (1997) Urinary 1-hydroxypyrene levels of garbage collectors with low-level exposure to polycyclic aromatic hydrocarbons. Sci Total Environ 199:159–164

    CAS  PubMed  Google Scholar 

  • Ichiba M, Wang Y, Oishi H, Iyadomi M, Shono N, Tomokuni K (1996) Smoking-related DNA adducts and genetic polymorphism for metabolic enzymes in human lymphocytes. Biomarkers 1:211–214

    CAS  Google Scholar 

  • Ichinose T, Yajima Y, Nagashima M, Takenoshita S, Nagamachi Y, Sagai M (1997) Lung carcinogenesis and formation of 8-hydroxy-deoxyguanosine in mice by diesel exhaust particles. Carcinogenesis 18:185–192

    Article  CAS  PubMed  Google Scholar 

  • International Agency for Research on Cancer (1984) Polynuclear aromatic compounds, part 3: Industrial exposures in aluminium production, coal gasification, coke production, and iron and steel founding. In: IARC monographs on the evaluation of the carcinogenic risk of chemicals in humans. Lyons, vol 34, pp 101–131

  • Jongeneelen FJ, van Leeuwen FE, Oosterink S, Anzion RBM, van der Loop F, Bos RP, van Veen HG (1990) Ambient and biological monitoring of coke-oven workers: determinants of the internal dose of polycyclic aromatic hydrocarbons. Br J Ind Med 47:454–461

    CAS  PubMed  Google Scholar 

  • Kasai H, Nishimura S, Kurokawa Y, Hayashi Y (1987) Oral administration of the renal carcinogen potassium bromate specifically produces 8-hydroxydeoxyguanosine in rat target organ DNA. Carcinogenesis 8:1959–1961

    CAS  PubMed  Google Scholar 

  • Kasai H, Chung MH, Jones DS, Inoue H, Ishikawa H, Kamiya H, Ohtsuka E, Nishimura S (1991) 8-Hydroxyguanine, a DNA adduct formed by oxygen radicals: its implication on oxygen radical-involved mutagenesis/carcinogenesis. J Toxicol Sci 16 [Suppl 1]:95–105

  • Kasai H, Iwamoto-Tanaka N, Miyamoto T, Kawanami K, Kawanami S, Kido R, Ikeda M (2001) Life style and urinary 8-hydroxydeoxyguanosine a marker of oxidative DNA damage: effects of exercise, working conditions, meat intake, body mass index, and smoking. Jpn J Cancer Res 92:9-15

    CAS  PubMed  Google Scholar 

  • Lagorio S, Tagesson C, Forastiere F, Iavarone I, Axelson O, Carere A (1994) Exposure to benzene and urinary concentrations of 8-hydroxydeoxyguanosine, a biological marker of oxidative damage to DNA. Occup Environ Med 51:739–743

    CAS  PubMed  Google Scholar 

  • Lodovici M, Casalini C, Cariaggi R, Michelucci L, Dolara P (2000) Levels of 8-hydroxydeoxyguanosine as a marker of DNA damage in human leukocytes. Free Radic Biol Med 28:13–17

    Article  CAS  PubMed  Google Scholar 

  • Marczynski B, Rozynek P, Elliehausen HJ, Korn M, Baur X (1997) Detection of 8-hydroxydeoxyguanosine, a marker of oxidative DNA damage in white blood cells of workers occupationally exposed to styrene. Arch Toxicol 71:496–500

    Article  CAS  PubMed  Google Scholar 

  • Marczynski B, Rihs HP, Rossbach B, Holzer J, Angerer J, Scherenberg M, Hoffmann G, Bruning T, Wilhelm M (2002) Analysis of 8-oxy-7,8-dihydro-2′-deoxyguanosine and DNA strand breaks in white blood cells of occupationally exposed workers: comparison with ambient monitoring, urinary metabolites and enzyme polymorphisms. Carcinogenesis 23:273–281

    Article  CAS  PubMed  Google Scholar 

  • Nakae D, Kobayashi Y, Akai H, Andoh N, Satoh H, Ohashi K, Tsutsumi M, Konishi Y (1997) Involvement of 8-hydroxyguanine formation in the initiation of rat liver carcinogenesis by low dose levels of N-nitrosodiethylamine. Cancer Res 57:1281–1287

    CAS  PubMed  Google Scholar 

  • Nakajima M, Takeuchi T, Takeshita T, Morimoto K (1996) 8-Hydroxydeoxyguanosine in human leukocyte DNA and daily health practice factors: effects of individual alcohol sensitivity. Environ Health Perspect 104:1336–1338

    CAS  PubMed  Google Scholar 

  • Nilsson RI, Nordlinder RG, Agesson C, Walles S, Jarvholm BG (1996) Genotoxic effects in workers exposed to low levels of benzene from gasoline. Am J Ind Med 30:317–324

    Article  CAS  PubMed  Google Scholar 

  • Redmond CK (1983) Cancer mortality among coke-oven workers. Environ Health Perspect 52:67–73

    CAS  PubMed  Google Scholar 

  • Tagesson C, Chabiuk D, Axelson O, Baranski B, Palus J, Wyszynska K (1993) Increased urinary excretion of the oxidative DNA adduct 8-hydroxydeoxyguanosine as a possible early indicator of occupational cancer hazards in the asbestos rubber and azo-dye industries. Pol J Occup Med Environ Health 6:357–368

    CAS  PubMed  Google Scholar 

  • Takahashi K, Pan G, Kasai H, Hanaoka T, Feng Y, Liu N, Zhang S, Xu Z, Tsyda T, Yamato H, Higashi T, Okubo T (1997) Relationship between asbestos exposure and 8-hydroxydeoxyguanosine levels in leukocytic DNA of workers at a Chinese asbestos-material plant. Int J Occup Environ Health 3:111–119

    PubMed  Google Scholar 

  • Toraason M, Hayden C, Marlow D, Rinehart R, Mathias P, Werren D, DeBord DG, Reid TM (2001) DNA strand breaks, oxidative damage and 1-OH pyrene in roofers with coal-tar pitch dust and/or asphalt fume exposure. Int Arch Occup Environ Health 74:396–404

    Article  CAS  PubMed  Google Scholar 

  • Yamaguchi R, Hirano T Asami S, Chung MH, Sugita A, Kasai H (1996) Increased 8-hydroxyguanine levels in DNA and its repair activity in rat kidney after administration of a renal carcinogen ferric nitrilotriacetate. Carcinogenesis 17:2419–2422

    CAS  PubMed  Google Scholar 

  • Yamano Y, Tokutake T, Haga M, Ishihara Y, Morinobu S, Kagawa J (1990) Determination of cotinine by high performance liquid chromatography and evaluation of exposure to tobacco smoke. Proceedings of 63rd Annual Meeting of Japan Association of Industrial Health (in Japanese), p126

  • Yamano Y, Kagawa J, Hanaoka T, Takahashi T, Kasai H, Tsugane S, Watanabe S (1995) Oxidative DNA damage induced by silica in vivo. Environ Res 69:102–107

    CAS  PubMed  Google Scholar 

  • van Zeeland AA, de Groot AJL, Hall J, Donato F (1999) 8-Hydroxydeoxyguanosine in DNA from leukocytes of healthy adults: relationship with cigarette smoking, environmental tobacco smoke, alcohol, and coffee consumption. Mutat Res 439:249–257

    PubMed  Google Scholar 

Download references

Acknowledgments

We are grateful to D. Guan (Angang Public Health and Anti-Epidemic Station, China) and G. Gao (Angang Occupational Hygiene Institute, China) for their help with the epidemiological survey and sampling. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture, Japan.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Ichiba.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, J., Ichiba, M., Hanaoka, T. et al. Leukocyte 8-hydroxydeoxyguanosine and aromatic DNA adduct in coke-oven workers with polycyclic aromatic hydrocarbon exposure. Int Arch Occup Environ Health 76, 499–504 (2003). https://doi.org/10.1007/s00420-003-0449-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00420-003-0449-2

Keywords

Navigation