Abstract
In an attempt to define the pattern of developmental expression of AP-2rep and AP-2 in Xenopus embryos, we cloned a Xenopus AP-2rep cDNA. The AP-2rep message was localized in the organizer region at the gastrula stage whereas AP-2 was expressed ventro-laterally in the animal hemisphere. Later, AP-2rep was expressed in the entire neural tissue at the neurula stage while AP-2 was predominantly expressed in the cranial neural crest areas. The endogenous expression of AP-2 in the neural crest area was diminished by ectopic injection of AP-2rep RNA, suggesting a role for AP-2rep in the differentiation of neural tissues by restricting the expression of AP-2 in the Xenopus embryo.
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Acknowledgements
We thank Drs. T. Sargent, M. Taira and N. Papalopulu for supplying of plasmids (AP-2, En-2, and BF-1, respectively). This work was partly supported by a grant-in-aid from The Ministry of Education, Science and Culture of Japan (to M.M.).
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Gotoh, M., Izutsu, Y. & Maéno, M. Complementary expression of AP-2 and AP-2rep in ectodermal derivatives of Xenopus embryos. Dev Genes Evol 213, 363–367 (2003). https://doi.org/10.1007/s00427-003-0336-6
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DOI: https://doi.org/10.1007/s00427-003-0336-6