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Ultrasensitive electrochemiluminescent immunoassay for morphine using a gold electrode modified with CdS quantum dots, polyamidoamine, and gold nanoparticles

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Abstract

We report on a novel electrochemiluminescent (ECL) immunoassay for the ultrasensitive determination of morphine by making use of a gold electrode which was modified with a nanocomposite film containing self-assembled polyamidoamine (PAMAM) CdS quantum dots and electrodeposited gold nanoparticles (Au-NPs). The highly uniform and well-dispersed quantum dots were capped with PAMAM dendrimers. Due to the synergistic effect of the modified quantum dots and the electrodeposited Au-NPs, the ECL response is dramatically enhanced. Under optimal experimental conditions, the immunoreaction between morphine and anti-morphine antibody resulted in a decrease of the ECL signal because of steric hindrance. The calibration plot is linear in the morphine concentration range from 0.2 to 180 ng•mL−1, with a detection limit as low as 67 pg•mL−1. The sensor was successfully applied to the determination of morphine in blood plasma. This kind of assay is expected to pave new avenues in label-free drug assays.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 21075086, 21075087), Natural Science Foundation of Jiangsu Province (BK2011273), the Project of Scientific and Technologic Infrastructure of Suzhou (SZS201207), and the Open Research Project of the Battery Technology Innovation Center for Public Services of Jiangsu Province (HDP201206).

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Materials Science; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Soochow University, Suzhou, 215123, Peoples Republic of China

    Wenjuan Fei, Feifei Chen, Li Sun, Qianhua Li & Ying Wu

  2. Department of Anesthesiology of First Affiliated Hospital of Soochow University, Suzhou, 215006, Peoples Republic of China

    Jianping Yang

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  1. Wenjuan Fei
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  2. Feifei Chen
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  3. Li Sun
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  4. Qianhua Li
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  5. Jianping Yang
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  6. Ying Wu
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Corresponding authors

Correspondence to Jianping Yang or Ying Wu.

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Fei, W., Chen, F., Sun, L. et al. Ultrasensitive electrochemiluminescent immunoassay for morphine using a gold electrode modified with CdS quantum dots, polyamidoamine, and gold nanoparticles. Microchim Acta 181, 419–425 (2014). https://doi.org/10.1007/s00604-013-1130-4

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  • DOI: https://doi.org/10.1007/s00604-013-1130-4

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