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Variibacter gotjawalensis gen. nov., sp. nov., isolated from soil of a lava forest

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Abstract

A novel bacterial strain designated GJW-30T was isolated from soil of the lava forest, Gotjawal, located in Aewol, Jeju, Korea. Strain GJW-30T was found to be strictly aerobic, Gram-negative and to form pleomorphic, non-motile rods and white colonies on R2A agar. The major fatty acids were identified as C18:1ω7c, C16:0 and C17:0, the predominant isoprenoid quinone as Q-10, the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid. The cell-wall sugar pattern of strain GJW-30T was found to be composed of glucose, ribose and rhamnose and meso-DAP as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain GJW-30T is 62.2 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain GJW-30T forms a deep branch within the order Rhizobiales, sharing the highest level of sequence homology with Bradyrhizobium oligotrophicum LMG 10732T (93.6 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain GJW-30T is considered to represent a novel genus and species, for which the name Variibacter gotjawalensis gen. nov., sp. nov. (the type strain is GJW-30T = KCTC 32391T = CECT 8514T = LMG 28093T) is proposed.

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Acknowledgments

This work was supported by Mid-career Researcher Program through NRF grant funded by the Ministry of Science, ICT and Future Planning (MSIFP) of the Republic of Korea and a grant from the KRIBB Research Initiative Program. I thank Professors J. P. Euzeby and Bernhard Schink for his advice in naming the novel strain.

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Authors and Affiliations

  1. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Kwang Kyu Kim, Keun Chul Lee, Mi Kyung Eom & Jung-Sook Lee

  2. Gyeongbuk Institute for Marine Bioindustry, Uljin, 767-813, Republic of Korea

    Jong-Shik Kim

  3. Research Institute for Hallasan, Jeju-si, Jeju Special Self-Governing Province, 690-816, Republic of Korea

    Dae-Shin Kim & Suk-Hyung Ko

  4. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Byung-Hyuk Kim

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  1. Kwang Kyu Kim
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Correspondence to Jung-Sook Lee.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Variibacter gotjawalensis GJW-30T is HF970589.

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Kim, K.K., Lee, K.C., Eom, M.K. et al. Variibacter gotjawalensis gen. nov., sp. nov., isolated from soil of a lava forest. Antonie van Leeuwenhoek 105, 915–924 (2014). https://doi.org/10.1007/s10482-014-0146-z

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