Responses to selection of S₅ inbreds for broad-based resistance to ear rots and grain mycotoxin contamination caused by Fusarium spp. in maize

Abstract

Random S₅ inbreds derived from three F₂ maize (Zea mays L.) populations (L1934 × LP918, LP915 × LP2541 and L7310 × L7266) were selected for ear rot resistance after inoculation with a low-fumonisin producing isolate belonging to F. proliferatum. The four less susceptible and the four most susceptible inbreds from each population were crossed and F₁ seeds were pooled. Resistant and susceptible pools from each population were evaluated for disease severity (percentage of the ear visibly diseased) after inoculation with the isolate used for selection, and high toxigenic isolates belonging to F. verticillioides and F. graminearum. Grain mycotoxin concentration was assessed by ELISA. Differences in disease resistance to each fungus were observed between resistant and susceptible pools in most populations and environments indicating that selection after inoculation with a single species might be effective to develop broad-based resistance to Fusarium. Resistant pools exhibited, after inoculation with F. verticillioides, low grain fumonisin concentrations in most populations and years. Positive genotypic correlations between disease severity and fumonisin concentration (0.89 < r_g < 0.98, depending on fungal species and year) indicate that selection for disease severity accounted for most of the variability for field fumonisin accumulation. Selection seemed to be also effective to reduce grain deoxynivalenol and zearalenone concentrations after inoculation with F. graminearum. Ratios between grain deoxynivalenol concentration and disease severity were lower in L7310 × L7266 than those observed in the other populations suggesting that mechanisms affecting mycotoxin accumulation might exist in this population and additional responses should be feasible if including deoxynivalenol concentration as another selection parameter.