Scientific paper
HLA-DR antigen expression on peripheral blood monocytes correlates with surgical infection

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Abstract

Monocyte human leukocyte antigen-DR (HLA-DR) expression has correlated closely with clinical outcome in severely injured patients at high risk for infection. Monocytes from 77 asymptomatic volunteers expressed HLA-DR antigen with minimal variability in respect to age, gender, race, time of day or year, or serum alcohol level. Patients who developed infection after elective laparotomy had a significantly lower mean percentage of monocytes expressing HLA-DR antigen and a lower mean fluorescent intensity than uninfected patients (p < 0.05). Severely infected nonsurgical patients had significantly lower values than normal volunteers (p < 0.01), and the mean fluorescent intensity of those who died from infection was significantly lower than that of those who survived (p < 0.05). Patients on immunosuppressive regimens after renal transplantation had levels of HLA-DR expression similar to those of the volunteers. Monocyte HLA-DR expression was found to be a reliable marker of clinical infection and showed remarkable reproducibility within the normal uninfected study population.

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    Supported in part by the John W. and Caroline Price Foundation, Price Institute of Surgical Research, University of Louisville School of Medicine, Louisville, Kentucky.

    1

    From the Department of Surgery and Price Institute of Surgical Research, University of Louisville School of Medicine, Louisville, Kentucky.

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