A sensitive and continuous fluorometric assay for phospholipase A2 using pyrene-labeled phospholipids in the presence of serum albumin
References (27)
- et al.
- et al.
Biochem. Pharmacol
(1976) - et al.
- et al.
- et al.
Bioorg. Chem
(1976) - et al.
J. Lipid Res
(1983) - et al.
Anal. Biochem
(1981) - et al.
Biochem. Biophys. Res. Commun
(1981) - et al.
Chem. Phys. Lipids
(1983)
J. Lipid Res
(1975)
Biochim. Biophys. Acta
(1973)
J. Lipid Res
(1969)
Cited by (240)
A non-venomous sPLA<inf>2</inf> of a lepidopteran insect: Its physiological functions in development and immunity
2018, Developmental and Comparative ImmunologyToll immune signal activates cellular immune response via eicosanoids
2018, Developmental and Comparative ImmunologySpecific inhibition of Xenorhabdus hominickii, an entomopathogenic bacterium, against different types of host insect phospholipase A<inf>2</inf>
2017, Journal of Invertebrate PathologyThe role of pilin protein of Xenorhabdus nematophila against immune defense reactions of insects
2017, Journal of Insect PhysiologyNeurotoxic phospholipase A<inf>2</inf> from rattlesnake as a new ligand and new regulator of prokaryotic receptor GLIC (proton-gated ion channel from G. violaceus)
2016, ToxiconCitation Excerpt :Purified CBd or CBd-GLIC complex were used for investigation of the steady-state enzyme kinetics. Steady-state kinetic parameters for CB were determined by spectrofluorometric assay (Radvanyi et al., 1989) using the standard reaction mixture with varying substrate concentrations (0.5–7.5 μM β-py-C10-PG). The values of Vmax and Km were calculated from a Lineweaver-Burk plot (double-reciprocal plot).
- 1
Present address: North Carolina A and T State University, Department of Chemistry, Greensboro, NC 27411. Recipient of a Chateaubriand Fellowship from the French Ministry of Foreign Affairs.
Copyright © 1989 Published by Elsevier Inc.