Purification of xylanase, β-glucosidase, endocellulase, and exocellulase from a thermophilic fungus, Thermoascus aurantiacus

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Abstract

A strain of thermophilic fungus, Thermoascus aurantiacus, was isolated from local soil. From the culture filtrates of the organism grown on blotting paper, a xylanase, β-glucosidase, exocellulase, and endocellulase were obtained in large amounts in highly purified form by employing ion-exchange and gel-permeation chromatography. The xylanase was crystallized. The xylanase and endocellulase were stable at 70 °C for 8 h, whereas the β-glucosidase and exocellulase were less stable at 70 °C.

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Present address: Rockefeller University, New York, NY 10021.

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