A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules

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Abstract

The recently developed technique of two-dimensional (2D) cross-relaxation spectroscopy is utilized for systematic measurements of selective nuclear Overhauser enhancements (NOE) in the high resolution 1H nuclear magnetic resonance (NMR) spectra of biological macromolecules in solution. Compared to conventional one-dimensional NOE studies, the 2D NOE experiment has the principal advantage that it avoids detrimental effects arising from the limited selectivity of preirradiation in crowded spectral regions. Furthermore, it yields with a single instrument setting a complete network of NOE's between all the protons in the macromolecule. The resulting information on intramolecular proton-proton distances provides a new avenue for studies of the spatial structures of biopolymers.

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      Citation Excerpt :

      Deconvolutions of CD spectra were obtained by BESTSEL software (http://bestsel.elte.hu/) [53]. Conformational studies were performed by registering NMR 1D [1H], 2D [1H, 1H] TOCSY (Total Correlation Spectroscopy) [37], NOESY (Nuclear Overhauser Enhancement Spectroscopy) [39], ROESY (Rotating frame Overhauser Enhancement Spectroscopy) [38] and DQFCOSY (Double Quantum-Filtered Correlated Spectroscopy) [54] spectra on a Varian Unity Inova 600 MHz spectrometer provided with a cold probe. Spectra were acquired with 16–64 scans, 128–256 FIDs in t1, 1024 or 2048 data points in t2, and with the following mixing times: 70 ms for TOCSY experiments, 200 and 300 ms for NOESY experiments, 250 ms for ROESY experiments.

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    On leave from the Department of Physics, Indian Institute of Science, Bangalore, India.

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