Astrocytes possess prostaglandin F receptors coupled to phospholipase C

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Abstract

We examined the effect of prostaglandin (PG) F on phosphoinositide (PI) hydrolysis in rat cultured astrocytes. PGF stimulated the formation of [3H]inositol phosphates in [3H]inositol-labeled astrocytes with the ED50 value of 23 nM, whereas PGD2 and PGE2 were much less effective than PGF. Transformation of astrocytes was accompanied by an increase in the stimulatory response of PGF. Pretreatment of the astrocytes with pertussis toxin and cholera toxin did not affect the PGF-evoked PI hydrolysis. In the digitonin-permeabilized astrocytes, PGF significantly enhanced the GTPγS-evoked PI hydrolysis in the presence of Ca2+. These results indicate that rat cultured astrocytes possess PGF receptors coupled to phospholipase C.

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