Ultrastructural analysis of the serotonin hyperinnervation in adult rat neostriatum following neonatal dopamine denervation with 6-hydroxydopamine

doi:10.1016/0006-8993(92)90363-E

Brain Research

Volume 569, Issue 1, 8 January 1992, Pages 1-13

https://doi.org/10.1016/0006-8993(92)90363-E Get rights and content

Abstract

Serotonin (5-HT) immunocytochemistry was used at the electron microscopic level to characterize the ultrastructural features of 5-HT axon terminals (varicosities) hyperinnervating the neostriatum of adult rats, 3 months after a neonatal destruction of the nigrostriatal dopamine system by intraventricular 6-hydroxydopamine. 5-HT-immunostained terminals from the anterior half of the hyperinnervated neostriatum were examined in single thin sections, and compared to their counterparts in vehicle-injected controls with respect to shape, size, organelle content, presence of a synaptic membrane differentiation and composition of the microenvironment. The intrinsic and relational features of the 5-HT-immunostained varicosities were essentially the same in 5-HT-hyperinnervated and control tissue. In particular, the frequency with which these varicosities made synaptic contacts was similarly low in both conditions (6–8% for whole varicosities), as already described in normal adult rat neostriatum. The distributional frequency of elements juxtaposed to the 5-HT-immunostained varicosities was also comparable in control and 5-HT-hyperinnervated tissue. However, in both conditions, there were much fewer dendritic spines in the microenvironment of 5-HT varicosities than around unlabeled terminals randomly selected from the same thin sections. This difference seemed entirely due to the numerous axo-spinous synaptic contacts made by the randomly selected, unlabeled varicosities. Together with recent observations on the 5-HT-hyperinnervation of adult rat hippocampus after grafts of fetal neurons, these data lead to the suggestion that mostly non-junctional neostriatal 5-HT terminals are not committed to a specific intratissular microenvironment. This might in part explain why they grow in excess when reinnervating adult tissue after a lesion or a graft.

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^☆: Presented in part at the Third IBRO World Congress of Neuroscience, Montréal, Canada, August 4–9, 1991.

^∗∗: Current address: Department of Pharmacology, University of Pennsylvania, John Morgan Building, 36 and Hamilton Walk, Philadelphia, PA 19104, U.S.A.

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Research reportUltrastructural analysis of the serotonin hyperinnervation in adult rat neostriatum following neonatal dopamine denervation with 6-hydroxydopamine☆

Abstract

Brain Research

Neuroscience

Brain Res. Bull.

Brain Res. Bull.

Brain Research

Brain Research

Brain Research

Brain Research

Brain Research

Dev. Brain Res.

Brain Research

Neuroscience

Brain Research

Brain Research

Brain Research

Brain Research

Neuroscience

Exp. Neurol.

Neuroscience

Brain Research

Neurosci. Lett.

Brain Research

Dev. Brain Res.

Brain Res. Bull.

Brain Res. Bull.

Brain Res.

Neuroscience

Brain Research

Brain Research

Brain Research

Brain Research

Brain Research

Brain Research

Brain Research

Brain Research

Brain Research

Pharmacol. Biochem. Behav.

Brain Res. Bull.

Brain Research

Neuroscience

Physiol. Behav.

Brain Research

Growth of transplanted monoaminergic neurons into the adult hippocampus along the perforant path

Nature

VIP neurons as prime synaptic targets for serotonin afferents in rat suprachiasmatic nucleus: a combined radioautographic and immunocytochemical study

J. Neurocytol.

Behavioral differences between neonatal and adult 6-hydroxydopamine-treated rats to dopamine agonists: relevance to neurological symptoms in clinical syndromes with reduced brain dopamine

J. Pharmacol. Exp. Ther.

Research report
Ultrastructural analysis of the serotonin hyperinnervation in adult rat neostriatum following neonatal dopamine denervation with 6-hydroxydopamine☆