NMR spectroscopic investigation of agarose oligomers produced by an α-agarase
References (23)
Proc. Int. Seaweed Symp.
(1966)Food Hydrocolloids
(1992)- et al.
Carbohydr. Res.
(1989) - et al.
Carbohydr. Res.
(1978) - et al.
Carbohydr. Polym.
(1992) - et al.
Adv. Carbohydr. Chem. Biochem.
(1983) Adv. Carbohydr. Chem. Biochem.
(1987)- et al.
Hydrobiologia
(1991) Bot. Mar.
(1984)
Carbohydr. Res.
Cited by (47)
Characterizing of a new α-agarase AgaE from Thalassomonas sp. LD5 and probing its catalytically essential residues
2022, International Journal of Biological MacromoleculesCitation Excerpt :13C NMR was recorded on an Agilent DD2 NMR at 500 MHz (Agilent Technologies, USA). NMR signals were assigned according to Rochas et al. [23]. The molecular weight of the purified oligosaccharides was detected as previously described using ESI-MS in negative mode [16].
Identification and biochemical characterization of a novel exo-type β-agarase Aga3463 from an Antarctic Pseudoalteromonas sp. strain
2019, International Journal of Biological MacromoleculesCitation Excerpt :GNUM-1 [19] and AgaH71 from Pseudoalteromonas hodoensis H7 [36]. The 13C NMR results for the structure of the agarolytic product also showed that Aga3463 could hydrolyze the β-1,4 linkages of agarose, but not the α-1,3 linkages [37,38]. In addition, the TLC and LC-MS results clearly showed that recombinant Aga3463 hydrolyzed agarose to neoagarobiose with no neooligosaccharide intermediates.
Monitoring and preparation of neoagaro- and agaro-oligosaccharide products by high performance anion exchange chromatography systems
2015, Carbohydrate PolymersCitation Excerpt :Two types of oligosaccharides can be derived from agarose by agarase or acid actions (Fig. 1). β-Agarase, the main agarase isolated from marine bacteria, would cleave the β-(1 → 4)-galactosidic bond of the polymer to release neoagaro-oligosaccharides (NAOS, i.e., various units of the neoagarobiose, 3,6-AG-α-(1 → 3)-Gal) (Malmqvist, 1978; Allouch, Helbert, Henrissat, & Czjzek, 2004; Henshaw et al., 2006), while the action of α-agarase or acids (e.g., HCl) would cleave the α-(1 → 3) bond to release agaro-oligosaccharides (AOS, i.e., various units of the agarobiose, Gal-β-(1 → 4)-3,6-AG) (Young, Bhattacharjee, & Yaphe, 1978; Rochas, Potin, & Kloareg, 1994). In recent years, bioactivity studies have demonstrated that the above oligomer forms derived from agar or agarose exhibit variety of physiological activities.
An extra peptide within the catalytic module of a β-agarase affects the agarose degradation pattern
2013, Journal of Biological ChemistryCitation Excerpt :Based on their cleaved linkage type, agarases are classified into two groups: α-agarases and β-agarases. The α-agarases (EC 3.2.1.158) degrade agarose at α1–3 linkages, producing agaro-oligosaccharides with β1-4–3,6-anhydro-l-galactopyranose as the reducing end (2), whereas the β-agarases (EC 3.2.1.81) degrade agarose at β1–4 linkages, producing neoagaro-oligosaccharides (NAOs) with α-1,3-d-galactose as the reducing end (3, 4). More than 50 agarases have been identified and characterized in different microorganisms to date (5–7).