Elsevier

Clinica Chimica Acta

Volume 18, Issue 3, December 1967, Pages 325-334
Clinica Chimica Acta

Electrophoresis and sudan black staining of lipoproteins on gelatinised cellulose acetate

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Abstract

Destaining of gelatinised cellulose acetate strips after electrophoresis of lipoproteins stained with Sudan Black B is demonstrated. This is achieved by diminishing the lipophilic character of the medium by a short treatment with alkali, which converts the cellulose acetate to cellulose, without affecting the lipoproteins. Four distinct fractions appear: pre-α1, which contains more than only non-esterified fatty acids; α1, α2 and β-lipoproteins without any trail. The so-called “chylomicrons” are for the most part absent and seem to migrate with the α2-globulins, like in free boundary electrophoresis. The lipid components of the different fractions are investigated by means of thin-layer chromatography. The results of lipoprotein electrophoresis are compared on this medium and on filter paper in health and disease.

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