A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies
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Reactive oxygen and nitrogen species in patients with rheumatoid arthritis as potential biomarkers for disease activity and the role of antioxidants
2016, Free Radical Biology and MedicineCitation Excerpt :Anti-CCP was considered positive at a serum concentration ≥5 IU/ml as reported by Kim et al. [24]. Serum MMP-3 was measured using Stromelysin-1-Kit (Fuji chemical Industries, LTD, Japan) by an enzyme immunoassay (EIA) system as described by Obata et al. [25]. The specific fluorogenic oxidation of hydroethidine (HE) to ethidium (E+) (Sigma-Aldrich, Seelze, Germany) has been used as a measure of superoxide anion as reported by Benov et al. [26].
Menaquinone-7 as a novel pharmacological therapy in the treatment of rheumatoid arthritis: A clinical study
2015, European Journal of PharmacologyCitation Excerpt :Serum intact osteocalcin (intact OC) was also measured by enzyme immunoassay (EIA) (Mitsubishi Yuka, Tokyo, Japan) according to reported methodology (Merle and Delmas, 1990). Serum MMP-3 was measured by an enzyme immunoassay (EIA) system using Stromelysin-1-Kit (Fuji chemical Industries, Ltd., Japan) (Obata et al., 1992). All the patients were followed up at Assiut University Hospitals, and a written consent was obtained from each patient.
Matrix Metalloproteinase 3/Stromelysin 1
2013, Handbook of Proteolytic EnzymesMolecular modeling of binding between amidinobenzisothiazoles, with antidegenerative activity on cartilage, and matrix metalloproteinase-3
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