Elsevier

Clinica Chimica Acta

Volume 236, Issue 2, 15 May 1995, Pages 173-180
Clinica Chimica Acta

Research communication
Selenium dependent glutathione peroxidase activity in human follicular fluid

https://doi.org/10.1016/0009-8981(95)98130-9Get rights and content

Abstract

The purpose of this study was to evaluate the follicular fluid selenium status of in vitro fertilization patients in relation to their individual characteristics and treatment outcome. A total of 135 samples of follicular fluid were collected from 112 patients during transvaginal oocyte retrieval. Selenium levels and glutathione peroxidase (GSHPx) activity were measured in follicular fluid (FF) and blood serum. A selenium dependent GSHPx activity in follicular fluid has been demonstrated. Patients with unexplained infertility had significantly decreased follicular selenium levels as compared with those with tubal infertility or male factor. The mean GSHPx activity in follicles yielding oocytes which were subsequently fertilized exceeded that of the follicles with non-fertilized oocytes. Tobacco smoking resulted in significantly diminished follicular GSHPx activity. The Se/GSHPx pattern of the FF seems to be significant for the evaluation of the follicular milieu.

References (28)

  • J.G. Alvarez et al.

    Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation

    Gamete Res

    (1989)
  • T. Miyazaki et al.

    Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

    J Reprod Fertil

    (1991)
  • R.I. Aitken et al.

    Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia

    J Androl

    (1989)
  • B. Frei et al.

    Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma. Protective effects of ascorbic acid

    Biochem J

    (1991)
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