Brief communicationHydroxyethyl starch as a cryopreservative for nucleated mammalian cells☆
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Cited by (14)
The effect of hydroxyethyl starch as a cryopreservation agent during freezing of mouse pancreatic islets
2024, Biochemistry and Biophysics ReportsOptimizing the transport and storage conditions of current Good Manufacturing Practice –grade human umbilical cord mesenchymal stromal cells for transplantation (HUC-HEART Trial)
2019, CytotherapyCitation Excerpt :Due to its physical properties and depending on the concentration and molecular weight, HES influences the viscosity of solutions and decreases the cooling rate required for optimal survival during vitrification, increases propensity for supercooling and kinetically inhibits ice formation [25]. It was suggested that 10–20% HES was effective in the survival of cryopreserved cells [26]. Besides use as a CPA for many cell types, HES (6%) has also been used in human BM stem cells, resulting in higher cell recovery and cell viability compared with cells preserved in 10% DMSO [27].
Hydroxyethylstarch in cryopreservation - Mechanisms, benefits and problems
2012, Transfusion and Apheresis ScienceCitation Excerpt :Although a 6% HES concentration was used for plasma expansion, Knorpp selected a 15% solution without giving a reason for the chosen concentration. Indeed, when Persidsky [56] later compared HES to DMSO in the cryopreservation of rat bone marrow cells, 15% HES was found to be more effective than 10% or 20%. Ashwood-Smith et al. [57] found 10% and 15% HES equally effective compared to 10% DMSO in survival of cryopreserved Chinese hamster cells.
Chemical modification of starch
2004, Advances in Carbohydrate Chemistry and BiochemistryLa congélation de moelle osseuse
1975, Revue Francaise de Transfusion et Immuno-hematologie
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Supported by Contract N00014-68-C-0367 from the Office of Naval Research.
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Present address: The Institute of Medical Sciences, Pacific Medical Center, San Francisco, CA 94115.