Elsevier

Cryobiology

Volume 8, Issue 6, December 1971, Pages 586-588
Cryobiology

Brief communication
Hydroxyethyl starch as a cryopreservative for nucleated mammalian cells

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    Due to its physical properties and depending on the concentration and molecular weight, HES influences the viscosity of solutions and decreases the cooling rate required for optimal survival during vitrification, increases propensity for supercooling and kinetically inhibits ice formation [25]. It was suggested that 10–20% HES was effective in the survival of cryopreserved cells [26]. Besides use as a CPA for many cell types, HES (6%) has also been used in human BM stem cells, resulting in higher cell recovery and cell viability compared with cells preserved in 10% DMSO [27].

  • Hydroxyethylstarch in cryopreservation - Mechanisms, benefits and problems

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    Although a 6% HES concentration was used for plasma expansion, Knorpp selected a 15% solution without giving a reason for the chosen concentration. Indeed, when Persidsky [56] later compared HES to DMSO in the cryopreservation of rat bone marrow cells, 15% HES was found to be more effective than 10% or 20%. Ashwood-Smith et al. [57] found 10% and 15% HES equally effective compared to 10% DMSO in survival of cryopreserved Chinese hamster cells.

  • Chemical modification of starch

    2004, Advances in Carbohydrate Chemistry and Biochemistry
  • La congélation de moelle osseuse

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Supported by Contract N00014-68-C-0367 from the Office of Naval Research.

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Present address: The Institute of Medical Sciences, Pacific Medical Center, San Francisco, CA 94115.

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