Elsevier

Developmental Biology

Volume 24, Issue 1, January 1971, Pages 106-118
Developmental Biology

Patterns of RNA synthesis in regenerating limbs of the adult newt,Triturus viridescens

https://doi.org/10.1016/0012-1606(71)90049-2Get rights and content

Abstract

Polyacrylamide gel electrophoresis was used to investigate patterns of RNA synthesis in regenerating limbs of the adult newt. The general pattern of synthesis is one in which uridine-3H incorporation into 28 S, 18 S, 5 S, 4 S, and high molecular weight RNA increases over the periods of dedifferentiation, accumulation, and proliferation of blastema cells to a maximum during formation of the anlagen of the new limb parts. Once the anlagen have been laid down, synthesis of these RNA fractions, with the exception of high molecular weight RNA, decreases substantially. On the other hand, heterogeneous RNA appears to be synthesized at unchanging levels between the day 10 of regeneration and anlagen formation. The application of this system to future investigations is discussed.

Reference (23)

  • DarnellJ.E.

    Ribonucleic acids from animal cells

    Bacteriol. Rev.

    (1968)
  • Cited by (0)

    Supported by a Grant-in-Aid from the Society of Sigma Xi and by a Biomedical Sciences Grant from the School of Life Sciences, University of Illinois, to David L. Stocum.

    View full text