Elsevier

Developmental Biology

Volume 41, Issue 2, December 1974, Pages 391-394
Developmental Biology

Brief note
A simple procedure for the long-term cultivation of chicken embryos

https://doi.org/10.1016/0012-1606(74)90316-9Get rights and content

Abstract

A method is described which permits the growth of chicken embryos in petri dishes from the third to the 20th day of incubation. The procedure is relatively simple and has the advantage of providing ready access to the embryo and its membranes for tissue grafting, for introduction of teratogenic agents, and for microscopic observation of morphogenesis and growth.

References (10)

  • J.H. Elliott et al.

    Growth of chick embryos in polyethylene bags

    Poultry Sci

    (1971)
  • R. Auerbach et al.

    Tumor angiogenesis: Lack of inhibition by radiation

    (1974)
  • M.A. Corner et al.

    Extended survival of the chick embryo in vitro

    Experientia

    (1973)
  • J. Folkman

    Tumor angiogenesis

    Advan. Cancer Res

    (1974)
  • D. Knighton et al.

    Study of the avascular and vascular phases of tumor growth in the chick embryo

    (1974)
There are more references available in the full text version of this article.

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    Citation Excerpt :

    This study was approved by the research ethics committee of the Federal University of Goiás (Protocol number CEUA/UFG 056/2019). Angiogenic response to AZA was assessed using a CAM assay, based on the protocol described by Auerbach et al. (1974) with some modifications (Carneiro et al., 2016). Thirty fertilized hen eggs were incubated horizontally at 37 °C under 80% relative humidity in a BOD chamber (Model SL224).

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Supported by Grants Nos. CA-14019 and CA-13548 from the National Institutes of Health, Grant No. DT-2A from the American Cancer Society, and Grant No. 730-0597 from the Ford Foundation.

2

On leave from the Department of Zoology, University of Wisconsin, Madison, WI.

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