Cell reassociation behavior and lectin-induced agglutination of embryonic mouse cells from different brain regions

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Abstract

Cells from dissociated embryonic mouse cerebellum, when plated at high cell density in a 10 μl microwell, assembled into a reproducible three-dimensional pattern of reaggregates containing 2–10000 cells each and interconnecting cables of processes. The details of this pattern were modifiable by coating the culture substratum with poly-d-lysine and by the use of different types of serum in the growth medium. Formation of interconnecting cables was inhibited reversibly by the dimeric lectin Succinyl Concanavalin A (Succ-ConA). Cells from cerebellum, midbrain, medulla and cerebral cortex, cultured separately in microwells, formed distinguishable patterns that appeared to be more region-specific than developmental stage-specific.

With lectins as probes of carbohydrate-containing surface macromolecules, cells harvested from different tissue regions had different cell surface properties. In addition the concentration of concanavalin A (ConA) or wheat germ agglutinin (WGA) required for half maximal agglutination was greatly increased for cerebellar cells harvested later than embryonic day 16. These observations suggest both regional and age-specific alterations in cell surface elements required for lectin-induced agglutination during mouse brain development.

References (35)

  • RL Sidman et al.

    Exp neurol

    (1959)
  • IL Miale et al.

    Exp neurol

    (1961)
  • PG Nelson et al.

    Brain res

    (1973)
  • GR Delong et al.

    Dev biol

    (1970)
  • AMC Rapin et al.

    Adv cancer res

    (1974)
  • MM Burger
  • GL Nicolson et al.

    Biochem biophys acta

    (1972)
  • PC Letourneau

    Dev biol

    (1975)
  • SJ Kleinschuster et al.

    Exp cell res

    (1972)
  • RL Sidman et al.

    Brain res

    (1975)
  • Boulder Committee

    Anat rec

    (1970)
  • E Taber-Pierce

    Progr brain res

    (1973)
  • JB Angevine et al.

    Nature

    (1961)
  • VS Caviness et al.

    J comp neurol

    (1973)
  • GR Delong et al.

    J comp neurol

    (1962)
  • M del Cerro et al.

    J neurocytol

    (1976)
  • SM Crain

    Neurophysiologic studies in tissue culture

    (1976)
  • Cited by (0)

    This work was supported by an NIH postdoctoral fellowship No. 5 F32N505271-02 (M. E. H.) and by NIH RO1-NS-1123705.

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