Journal of Molecular Biology
Multiple origin usage for DNA replication in sdrA(rnh) mutants of Escherichia coli K-12: Initiation in the absence of oriC☆
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Cited by (89)
R-loop-dependent replication and genomic instability in bacteria
2019, DNA RepairCitation Excerpt :Nevertheless, these studies allowed the identification of four to five main putative and some minor oriKs. The major peak was found to be located in the Ter region, as shown in the initial study by de Massy et al. [66]. Interestingly, similar peaks were identified in topA topB null cells not overproducing RNase HI, however the Ter located peak was much higher in these cells compared to rnhA cells [78].
RNase HII Saves rnhA Mutant Escherichia coli from R-Loop-Associated Chromosomal Fragmentation
2017, Journal of Molecular BiologyCitation Excerpt :The rnhA mutants in E. coli show several phenotypes: slower growth, synthetic lethality with various DNA metabolism defects (recBCD, rep, recG, topA), and initiation of unscheduled replication away from the origin and especially in the terminus region (the so-called “constitutive stable DNA replication”) [24,38–40]. Alternative initiations in the rnhA mutants are robust enough to support replication of the entire bacterial chromosome under conditions of blocking replication from oriC [41,42]. In contrast, bacterial RNase HII is a very different enzyme, as in the presence of the physiological cation Mg2 + it shows almost no (~ 0.25%) RNase HI activity toward the RNA strand of RNA:DNA hybrids [43,44], but specifically cleaves the rN/dN transition within a DNA strand, equally attacking both R-patches and R-tracts and leaving a single rN on the 5′ end of the cleavage [45,46] (Fig. 1A).
RecBCD is required to complete chromosomal replication: Implications for double-strand break frequencies and repair mechanisms
2015, DNA RepairCitation Excerpt :Additionally, the ter sites on the chromosome are oriented in a manner that allows up to a tenth of the genome to over-replicate, a fact that is readily observed in the absence of RecD, and can beexaggerated under conditions of stress or in the presence of other mutations [66–69]. Furthermore, illegitimate initiations of replication are common events occurring at single-strand nicks or gaps, D-loops, and R-loops throughout the genomes of both prokaryotes and eukaryotes [69–74]. Both replication forks bypassing each other and illigitimate initiation events in previously replicated regions would create a third copy of genomic material in this region, leading to the presence of kilobases of DNA that must be degraded and resolved back to the point where each sequence is represented by exactly two copies.
Conformational preferences underlying reduced activity of a thermophilic ribonuclease H
2015, Journal of Molecular Biology
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A major part of this work was performed during the good summer of 1983 when T.K. visited the laboratory of Jean-Michel Louarn in Toulouse. This work was supported by a grant from the Action Thématique Programmée, CNRS-INRA “Microbiologie” no. 9575 and by grants from the National Institutes of Health (GM22092 and MBS grant PR08139) and a grant-in-aid for research from University of New Mexico to T.K.
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Present address: Laboratory of Molecular Genetics, National Institutes of Health, Bethesda, MD 20205, U.S.A.