Journal of Molecular Biology
Molecular substructure of a viral receptor-recognition protein: The gp17 tail-fiber of bacteriophage T7☆
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2018, Journal of Virological MethodsCitation Excerpt :As a result, there is a potential discrepancy between phages enumerated by plaque assay and the actual number of phages, including non-infective or prematurely degraded phage. Even though T7 and M13 phages are generally stable at a broad range of pH and temperature, their ability to infect host bacteria can deteriorate during amplification, high-speed centrifugation or long term storage (Rakonjac et al., 2011; Steven et al., 1988). Also, the plaque assay is time-consuming (up to ∼ 18–24 h) and can have variable reproducibility.
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Work at Brookhaven National Laboratory was supported by the Office of Health and Environmental Research of the U.S. Department of Energy.
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Permanent address: Department of Physics and Biophysics, Massey University, Palmerston North, New Zealand.