Elsevier

Life Sciences

Volume 40, Issue 11, 16 March 1987, Pages 1063-1070
Life Sciences

Determination of serotonin in whole blood, platelet-rich plasma, platelet-poor plasma and plasma ultrafiltrate

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Abstract

The important biogenic amine, serotonin (5HT), was determined in whole blood, platelet-rich plasma (PRP), platelet-poor plasma (PPP), and plasma ultrafiltrate after simple deproteinization. Following ion-pair chromatography on standard or narrow-bore reverse-phase columns, 5HT and the internal standard

(N-methylserotonin-NMS) were detected by flourometry with absolute detection limits of 2–4 pg. Levels obtained for whole blood and PRP were in agreement with previous methods. However, mean (±SD) values obtained for platelet-poor plasma (PPP) of 578±277 pg/ml (N=7) were approximately 3-fold lower than the lowest previous reports. We also report the first determination of 5HT in plasma ultrafiltrate, having observed mean levels of 387±222 pg/ml (N=7).

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