Death of rhizobia on inoculated seed

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Abstract

The death of R. trifolii WU1 and R. meliloti WU96 was studied with and without protection during the first hour after inoculation on mung bean (Vigna radiata) seeds. For unprotected rhizobia, death occurred in four distinct phases: a first phase of relatively slow death rate while the seeds remained moist; a second phase of very rapid death shortly after loss of visible moisture on seed; a third phase where numbers tended to stabilize, and a fourth phase in which a significant death rate continued. The use of protectant greatly reduced the overall death rate, especially the rapid death following loss of visible moisture. Some possible mechanisms of cell death and protection are discussed.

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