Elsevier

Virology

Volume 58, Issue 1, March 1974, Pages 164-175
Virology

Production of defective MS2 virions in resting Escherichia coli

https://doi.org/10.1016/0042-6822(74)90151-2Get rights and content

Abstract

Starvation of Escherichia coli for nitrogen, glucose, or sulfur was initiated by cellular consumption of the nutrient in a medium designed to contain only a limiting amount of nitrogen, glucose, or sulfur. MS2 infection of nitrogen-starved cells resulted in a normal yield of plaque-forming units. MS2 infection of sulfur-starved or glucose-starved E. coli, however, resulted in a 100-fold decrease in the yield of plaque-forming units. Further investigation of MS2 infection in sulfur-starved E. coli showed the major cause of the decrease in yield of plaque-forming units was the synthesis of noninfective virions.

References (39)

1

Present address: Miles Laboratories, Inc., 400 Morgan Lane, West Haven, Connecticut 06516.

2

Present address: Biophysics Unit, Agricultural Research Council, Babraham, Cambridge, CB2 4AT, England.

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