Packaging of tRNA and 6 S stable RNA into bacteriophage MS2 particles produced in Escherichia coli treated with 5-fluorouracil

doi:10.1016/0042-6822(82)90079-4

Virology

Volume 119, Issue 1, May 1982, Pages 209-213

https://doi.org/10.1016/0042-6822(82)90079-4 Get rights and content

Abstract

Treatment with 5-fluorouracil is known to result in the production of defective particles of Escherichia coli bacteriophage MS2. The RNA extracted from these defective particles was analyzed by gel electrophoresis, blot hybridization, and aminoacylation. It was concluded that one to three molecules of 6 S stable RNA and a similar amount of tRNA derived from E. coli were packaged in the MS2 defective particle together with a broken viral RNA molecule.

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²: Present address: Department of Biochemistry, Kyushu University School of Medicine, Maidashi 3-1-1, Higashi-ku, Fukuoka 812, Japan.

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Short communicationPackaging of tRNA and 6 S stable RNA into bacteriophage MS2 particles produced in Escherichia coli treated with 5-fluorouracil

Abstract

J. Mol. Biol.

Virology

J. Mol. Biol.

J. Mol. Biol.

Cell

J. Mol. Biol.

Cell

FEBS Lett.

J. Biol. Chem.

Nature (London)

Short communication
Packaging of tRNA and 6 S stable RNA into bacteriophage MS2 particles produced in Escherichia coli treated with 5-fluorouracil