Elsevier

Virology

Volume 154, Issue 2, 30 October 1986, Pages 395-400
Virology

Short communication
Cell killing by ultraviolet-inactivated human immunodeficiency virus

https://doi.org/10.1016/0042-6822(86)90465-4Get rights and content

Abstract

Extensive cell killing and cytopathology were observed within 24 hr after exposure of a clonal cell line of human T-4 lymphocytes (RH9) to culture supernatants containing human immunodeficiency virus (HIV). Ultraviolet-irradiated HIV-containing culture fluids were also capable of killing RH9 cells and of inducing specific cytopathic effects which were indistinguishable from those induced by unirradiated virus-containing preparations. The uv-irradiated HIV was incapable of forming proviral DNA using the endogenous virion genomic RNA as a template. The RH9 cells persistently infected with HIV did not release soluble cytotoxic factors to account for the cell killing observed when culture supernatants were added to unifected RH9 cells. The fraction involved in cell killing had the hydrodynamic properties of a retrovirus. These results suggest that a virion component is responsible for cell killing by HIV.

References (27)

  • S. Rasheed et al.

    Virology

    (1986)
  • W.P. Rowe et al.

    Virology

    (1970)
  • T. Graf

    Virology

    (1972)
  • J.D. Hoelzer et al.

    Virology

    (1979)
  • J.L. Marvaldi et al.

    Virology

    (1978)
  • R.F. Garry et al.

    Virology

    (1981)
  • D. Klatzmann et al.

    Science

    (1984)
  • H.C. Lane et al.

    N. Engl. J. Med.

    (1985)
  • H.M. Temin et al.

    J. Virol.

    (1974)
  • H.M. Temin et al.
  • M. Popovic et al.

    Science

    (1984)
  • L. Montagnier et al.
  • F. Wong-Staal et al.

    Nature (London)

    (1985)
  • Cited by (53)

    • Inflammasomes in T cells

      2022, Journal of Molecular Biology
      Citation Excerpt :

      The other was a lytic form of cell death that was preceeded by cell swelling. The identification of the latter as a form of programmed cell death, dates back to a time when any programmed cell death was equated with apoptosis.83 The analysis of ex vivo specimen of lymph nodes from infected individuals suggested that the cell death triggered by HIV occurs primarily in non-productively infected bystander cells (not staining positive for viral RNA) rather than in productively infected cells.84

    • Possible Biomarkers for the Early Detection of HIV-associated Heart Diseases: A Proteomics and Bioinformatics Prediction

      2015, Computational and Structural Biotechnology Journal
      Citation Excerpt :

      We also could not conduct proteomics studies directly in HIV-infected individuals without first identifying protein profiles of cells infected by HIV alone, because most HIV-patients are co-infected with other pathogenic viruses and microorganisms that may produce factors similar to those induced by HIV. We therefore chose a genetically stable, single-cell-clone of a human T-cell line (RH9) [55] and infected it with a biologically cloned North American HIV-Clade B (HBX) in vitro. The differentially-regulated proteins expressed in HIV-infected and counterpart uninfected cells were evaluated at numerous time points from 1.5-, 3,6-, 12- and 24-h, followed by weekly, then 4–6 weeks samplings for 6–7 months post-infection.

    View all citing articles on Scopus
    View full text