Analysis of induced sputum to examine the effects of prednisone on airway inflammation in asthmatic subjects☆
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Cited by (154)
Corticosteroid resistance in asthma: Cellular and molecular mechanisms
2022, Molecular Aspects of MedicineCitation Excerpt :Inhaled GCs decreases IL-5, IL-8 and granulocyte-macrophage colony-stimulating factor (GM-CSF) protein levels in induced sputum compared to untreated asthma patients (Dente et al., 2010; Inoue et al., 1999). Prednisone (0.5 mg/kg/die for 6 days) decreased induced sputum ECP levels in stable asthma patients compared to placebo-treated subjects (Claman et al., 1994). Similar results were shown in another study where prednisolone (30mg/die for 2 weeks) decreased induced sputum ECP levels in stable asthma patients compared to placebo-treated asthmatics (Keatings et al., 1997).
The Precision Interventions for Severe and/or Exacerbation-Prone (PrecISE) Asthma Network: An overview of Network organization, procedures, and interventions
2022, Journal of Allergy and Clinical ImmunologyCitation Excerpt :Cellular and biochemical analyses of induced sputum samples collected from participants with and without asthma have revealed differences in markers of eosinophilic inflammation and bronchovascular permeability in a population with asthma.149 Similarly, sputum-induced samples have revealed the expected rise (following an antigen challenge) and fall (following a prednisone treatment) of markers of eosinophilic inflammation.150,151 Sputum cell counts in PrecISE are determined in a central core laboratory.
Asthma: Pathogenesis and Phenotypes
2015, Murray and Nadel's Textbook of Respiratory Medicine: Volume 1,2, Sixth EditionAsthma outcomes: Biomarkers
2012, Journal of Allergy and Clinical ImmunologyCitation Excerpt :Sputum cell counts are a well-validated method for assessing cellular inflammation of the airways. Validation studies have included comparison with cell counts obtained during bronchoscopy93 and studies done before and after interventions with corticosteroids,87 aeroallergens,84,85 and ozone,86 as well as repeated measures and safety studies.83,94 Inverse relationships have been observed between percentage of sputum eosinophils and FEV1, as well as between percentage of sputum eosinophils and PC20 to methacholine,73 but sputum eosinophils alone do not account for all of the variability in these lung function measures among individuals who have asthma; other factors also appear to have strong influences.
- ☆
Supported by Program Project grant HL 24136 from the National Institutes of Health.
- a
From the Department of Medicine, University of California, San Francisco.
- b
From the Cardiovascular Research Institute, University of California, San Francisco.
- 1
Drs. Fahy and Claman are supported by National Research Service Award grant no. HL07185.