Cell
Volume 10, Issue 4, April 1977, Pages 697-702
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Article
Mammalian cells with defective mitochondrial functions: a Chinese hamster mutant cell line lacking succinate dehydrogenase activity

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Abstract

A mutant cell line derived from Chinese hamster fibroblasts is described which is defective in oxidative energy metabolism. Glucose is continuously required in the medium. As a result of a block in the Krebs cycle, these cells are auxotrophs for carbon dioxide and asparagine. Several experiments support our conclusion that the mutant cells lack appreciable levels of succinate dehydrogenase activity. Other components of the electron transport chain appear to be fully functional, although there is the possibility that electron transport and oxidative phosphorylation are uncoupled.

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