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ArticleA control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the region
References (32)
- et al.
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes
Cell
(1978) - et al.
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system
Gene
(1977) - et al.
Synthesis and accumulation of low molecular weight RNA during embryogenesis of Xenopus laevis
J. Mol. Biol.
(1966) - et al.
The isolation and characterization of a second oocyte 5S DNA from Xenopus laevis
Cell
(1977) - et al.
Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences
Cell
(1976) - et al.
Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro
Cell
(1979) - et al.
The transcription of 5S DNA injected into Xenopus oocytes
Dev. Biol.
(1978) - et al.
Differential effect of cordycepin triphosphate and 9β-D arabinofuranosyladenine triphosphate on tRNA and 5S RNA synthesis in isolated nuclei
Biochim. Biophys. Acta
(1979) - et al.
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the region
Cell
(1980) - et al.
The use of thin acrylamide gels for DNA sequencing
FEBS Letters
(1978)
Interaction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends
J. Biol. Chem.
Identification of factors required for accurate transcription of 5S RNA genes
Carnegie Inst. Washington Yearbook
High-fidelity transcription of 5S DNA injected into Xenopus oocytes
Cloned single repeating units of 5S DNA direct accurate transcription of 5S RNA when injected into Xenopus oocytes
The in vitro transcription of 5S DNA
Determination of sequences in RNA
Cited by (343)
Pol III promoters to express small RNAs: Delineation of transcription initiation
2014, Molecular Therapy Nucleic AcidsCitation Excerpt :By purposely using H1 promoter to generate shRNA transcripts with different 5′ ends from a single construct, which will be processed by Dicer into distinct siRNA duplexes because Dicer can make a cleavage by measuring the distance from the 5′ ends,22 the chances of getting a potent shRNA will be significantly increased since one nucleotide change might change the potency dramatically, although the off-targets might also be increased due to multiple mature siRNAs might be generated. It has been commonly accepted that the termination signal for pol III promoter-driven transcription is a simple four to six-residue poly-T,44 which is currently used as the terminal signal for almost all small RNA expression cassettes driven by the pol III promoter. However, we found that poly-T might not be an efficient termination signal.
Structure, function and regulation of Transcription Factor IIIA: From Xenopus to Arabidopsis
2013, Biochimica et Biophysica Acta - Gene Regulatory MechanismsCitation Excerpt :This complex is required for RNA Polymerase III to initiate transcription accurately at the start site of the 5S rRNA gene. All known ICRs, except in yeast, consist of an A box extending from nucleotides + 50 to + 64 of the 120 bp-long 5S rRNA gene, an intermediate element (I.E. nt + 67 to + 72) and a C box (nt + 80 to + 97) defined in Xenopus leavis (Xl) [32–34] (Fig. 1A, Table 1). The ICR of the S. cerevisiae (Sc) 5S rRNA gene extends only from nucleotide + 81 to + 94 [35,36], corresponding to the distal C block element of the Xenopus 5S rRNA gene ICR.
Odd RNA polymerases or the A(B)C of eukaryotic transcription
2013, Biochimica et Biophysica Acta - Gene Regulatory MechanismsCitation Excerpt :One remembers that ten years after the discovery of multiple forms of RNA polymerase, the eukaryotic promoters were still proving elusive [19]. The characterization and mutagenesis of promoter elements were first done with simple genes such as 5S RNA and tRNA genes in M. Birnstiel, D. Brown, B. Hall and M. Smith laboratories [54–57]. These studies had shown that upstream sequences could be deleted without affecting transcription and that the “control regions” were, surprisingly, intragenic (one should recall that the concept of promoter derived from studies on prokaryotes conveyed with it the idea of RNA polymerase binding site).
Pure genes, pure genius
2012, CellCitation Excerpt :During a decade-long period straddling the advent of gene cloning, Brown and colleagues were able to develop a test tube system that accurately recapitulated 5S ribosomal RNA gene expression (Birkenmeier et al., 1978). This paved the way for the mapping of the regulatory sequences that facilitated 5S gene transcription, which, to the surprise of all, were located right in the middle of each gene (Bogenhagen et al., 1980). Finally, this path of research led to the first discovery of a eukaryotic transcription factor, designated TF3A (Pelham and Brown, 1980).
RNA polymerase III transcription control elements: Themes and variations
2012, GeneCitation Excerpt :As schematically represented in Fig. 2, primary transcripts with long 3′ trailers could first undergo processing by RNase Z, followed by further processing of the released trailer RNA by other RNA processing enzymes, as exemplified by the combined action of RNase Z and Dicer on some unusual Pol III-synthesized pri-miRNAs of murine γ-herpesvirus 68 (Bogerd et al., 2010). More than thirty years have passed since the first description of the gene-internal promoter elements of tRNA and 5S rRNA genes (Bogenhagen et al., 1980; Sakonju et al., 1980) (Galli et al., 1981; Hofstetter et al., 1981). A long series of later studies confirmed A and B boxes as the most distinctive control elements in Pol III transcription.
A tribute to the Xenopus laevis oocyte and egg
2004, Journal of Biological ChemistryCitation Excerpt :Two of us independently sequenced this construct to confirm this remarkable fact. Before specific RNA synthesis stopped, about one-third of the coding region had been replaced with plasmid sequences (54, 55). Deletions from the 3′ end altered termination as soon as one of the four Ts that encode the Us at the 3′ end of the mature 5 S RNA had been removed (56).