Gene amplification in the lac region of E. coli

doi:10.1016/0092-8674(84)90317-9

Cell

Volume 37, Issue 1, May 1984, Pages 217-224

https://doi.org/10.1016/0092-8674(84)90317-9 Get rights and content

Abstract

We have characterized strains of E. coli in which the lac region, together with varying amounts of surrounding DNA, is amplified 40 to 200 fold. The amplification events involve regions of 7 to 37 kb and result in a tandem array of repeated units. Restriction digest patterns of DNA from over 100 independent strains reveal that the amplified units are different in each case. Mechanisms of gene duplication and amplification, and the relationship of gene amplification in bacteria to that in eucaryotic cells, are considered.

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^§: Present address: Stanford University.

^∥: Present address: Istituto di Genetica, University of Pavia, Pavia, Italy.

^¶: Present address: University of California, Los Angeles.

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Cell

ArticleGene amplification in the lac region of E. coli

Abstract

Cell

J. Biol. Chem.

Meth. Enzymol.

J. Mol. Biol.

J. Mol. Biol.

J. Mol. Biol.

J. Mol. Biol.

J. Mol. Biol.

Cell

J. Mol. Biol.

J. Biol. Chem.

Tandem genetic duplications in phage and bacteria

Ann. Rev. Microbiol.

Spontaneous tandem genetic duplications in Salmonella typhimurium arise by unequal recombination between rRNA (rrn) cistrons

Structure of amplified DNA in different Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate

Mol. Cell. Biol.

Amplification of the metallothionein-1 gene in cadmium-resistant mouse cells

β-galactosidase chimeras: primary structure of a lac repressor-β-galactosidase protein

Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences

Specific gene amplification in oocytes

Science

The origin of human cancers

Nature

DNA sequence for a low-level promoter of the lac repressor gene and an “up” promoter mutation

Nature

Article
Gene amplification in the lac region of E. coli