A cellular gene encodes scrapie PrP 27-30 protein

doi:10.1016/0092-8674(85)90333-2

Cell

Volume 40, Issue 4, April 1985, Pages 735-746

https://doi.org/10.1016/0092-8674(85)90333-2 Get rights and content

Abstract

A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligo-nucleotide probes corresponding to the N terminus of the protein. Southern blotting with PrP cDNA revealed a single gene with the same restriction patterns in normal and acrapie-infected brain DNA. A single PrP-related gene was also detected in murine and human DNA. PrP-related mRNA was found at similar levels in normal and scrapie-infected hamster brain, as well as in many other normal tissues. Using antisera against PrP 27-30, a PrP-related protein was detected in crude extracts of infected brain and to a lesser extent in extracts of normal brain. Proteinase K digestion yielded PrP 27-30 in infected brain extract, but completely degraded the PrP-related protein in normal brain extract. No PrP-related nucleic acids were found in purified preparations of scrapie prions, indicating that PrP 27-30 is not encoded by a nucleic acid carried within the infectious particles.

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Cell

ArticleA cellular gene encodes scrapie PrP 27-30 protein

Abstract

Biochem. Biophys. Res. Commun.

Tetrahedron Lett.

Anal. Biochem.

Lancet

J. Mol. Biol.

J. Biol. Chem.

Meth. Enzymol.

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Tetrahedron Lett.

Cell

J. Biol. Chem.

Cell

Cell

Cell

J. Mol. Biol.

Anal. Biochem.

Biochim. Biophys. Acta

J. Comp. Pathol.

Does the agent of scrapie replicate without nucleic acid?

Nature

Purification of mouse immunoglobulin heavy-chain messenger RNA from total myeloma tumor RNA

Eur. J. Biochem.

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

Antibodies to a scrapie prion protein

Nature

Scrapie and Creutzfeldt-Jakob disease prion proteins share physical properties and antigenic determinants

Creutzfeldt-Jakob disease prion proteins in human brains

N. Engl. J. Med.

Identification of a protein that purifies with the scrapie prion

Science

Molecular characteristics of the major scrapie prion protein

Biochemistry

Scrapie PrP 27-30 is a sialoglycoprotein

J. Virol.

Complete nucleotide sequences of the T24 human bladder carinoma oncogene and its normal homologue

Nature

Rates of formation and thermal stabilities of RNA:DNA and DNA: DNA duplexes at high concentrations of formamide

Nucl. Acids Res.

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DNA

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Cell

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Viroids and prions

Scrapie infectivity, fibrils and low molecular weight protein

Nature

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Eur. J. Biochem.

Animal virus titration techniques

Unconventional viruses and the origin and dis-appearance of kuru

Science

Genbank (R) Release 23.0

Article
A cellular gene encodes scrapie PrP 27-30 protein