Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity

doi:10.1016/0092-8674(87)90168-1

Cell

Volume 50, Issue 7, 25 September 1987, Pages 1021-1029

https://doi.org/10.1016/0092-8674(87)90168-1 Get rights and content

Abstract

The phosphorylation of proteins on tyrosine in vivo and in vitro was examined in 3T3 cells stimulated by platelet-derived growth factor (PDGF) and transformed by polyoma middle T antigen (MTAg) by using an antibody directed against phosphotyrosine (P-tyr). Two common events were observed upon PDGF stimulation or MTAg transformation of cells: the appearance in the immunoprecipitates of an 85 kd phosphoprotein, and increased phosphatidylinositol (PI) kinase activity. In PDGF-stimulated cells, the 85 kd phosphoprotein and PI kinase activity appeared rapidly, within 1 min of growth factor addition. The PI kinase activity and 85 kd phosphorylation were also increased in anti-P-tyr immunoprecipitates from cells transformed by v-fms and v-sis, but not by SV40 T antigen. The presence of the tyrosine-phosphorylated 85 kd protein correlated with PI kinase activity during several purification steps. These results suggest that the 85 kd phosphoprotein, a putative PI kinase, is a substrate for both the PDGF receptor and MTAg/pp60^c-src tyrosine kinase activities.

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Cell

ArticleCommon elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity

Abstract

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J. Biol. Chem.

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Cell

Inositol trisphosphate formation and calcium mobilization in Swiss 3T3 cells in response to platelet-derived growth factor

Biochem. J.

Phosphoinositides and cell proliferation

J. Cell Sci.

Carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation

Altered sites of tyrosine phosphorylation in pp60c-src associated with polyoma-virus middle tumor antigen

Mol. Cell. Biol.

Recombinant retroviruses that transduce individual polyoma virus tumor antigens: effects on growth and differentiation

The complex of polyoma virus middle-T antigen and pp60c-src

EMBO J.

Purification of the platelet-derived growth factor receptor by using an anti-phosphotyrosine antibody

Protein kinase activities associated with distinct antigenic forms of polyoma virus middle-T antigen

EMBO J.

Simian sarcoma virus oncogene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor

Science

Characterization of a tyrosine-specific kinase activity in human fibroblast membranes stimulated by platelet-derived growth factor

J. Biol. Chem.

Localization of gene functions in polyoma virus DNA

Phosphatidylinositol turnover and transformation of cells by Abelson murine leukemia virus

EMBO J.

Article
Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity

Altered sites of tyrosine phosphorylation in pp60^c-src associated with polyoma-virus middle tumor antigen

The complex of polyoma virus middle-T antigen and pp60^c-src