An essential G1 function for cyclin-like proteins in yeast

doi:10.1016/0092-8674(89)90768-X

Cell

Volume 59, Issue 6, 22 December 1989, Pages 1127-1133

https://doi.org/10.1016/0092-8674(89)90768-X Get rights and content

Abstract

Cyclins were discovered in marine invertebrates based on their dramatic cell cycle periodicity. Recently, the products of three genes associated with cell cycle progression in S. cerevisiae were found to share limited homology with cyclins. Mutational elimination of the CLN1, CLN2, and $DAF1 WHI1$ products leads to cell cycle arrest independent of cell type, while expression of any one of the genes allows cell proliferation. Using strains where CLN1 was expressed conditionally, the essential function of Cln proteins was found to be limited to the G1 phase. Furthermore, the ability of the Cln proteins to carry out this function was found to decay rapidly upon cessation of Cln biosynthesis. The data are consistent with the hypothesis that Cln proteins activate the Cdc28 protein kinase, shown to be essential for the G1 to S phase transition in S. cerevisiae. Because of the apparent functional redundancy of these genes, $DAF1 WHI1$ has been renamed CLN3.

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^‡: Present address: Rockefeller University, New York, New York 10021.

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Cell

ArticleAn essential G1 function for cyclin-like proteins in yeast

Abstract

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J. Biol. Chem.

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J. Theor. Biol.

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Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules

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DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae

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Article
An essential G1 function for cyclin-like proteins in yeast

Involvement of cdc13⁺ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules

Cloning and sequencing of the cyclin related cdc13⁺ gene and a cytological study of its role in fission yeast mitosis