Identification of a major keratinocyte cell envelope protein, loricrin

doi:10.1016/0092-8674(90)90073-N

Cell

Volume 61, Issue 6, 15 June 1990, Pages 1103-1112

https://doi.org/10.1016/0092-8674(90)90073-N Get rights and content

Abstract

During epidermal cell cornification, the deposition of a layer of covalently cross-linked protein on the cytoplasmic face of the plasma membrane forms the cell envelope. We have isolated and characterized cDNA clones encoding a major differentiation product of mouse epidermal cells, which has an amino acid composition similar to that of purified cell envelopes. Transcripts of this gene are restricted to the granular layer and are as abundant as the differentation-specific keratins, K1 and K10. An antiserum against a C-terminal peptide localizes this protein in discrete granules in the stratum granulosum and subsequently at the periphery of stratum corneum cells. Immunofluorescence and immunoelectron microscopy detect this epitope only on the inner surface of purified cell envelopes. Taken together, these results suggest that it is a major component of cell envelopes. On the basis of its presumed function, this protein is named loricrin.

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^†: Present Address: Department of Dermatology, New York University, Medical Center, New York, New York 10016.

^∥: Present Address: Dermatologische Klinik, Universitatsspital, Gloriastrasse 31, CH-8091 Zurich, Switzerland.

^★★: Present Address: Laboratory of Skin Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892.

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Cell

ArticleIdentification of a major keratinocyte cell envelope protein, loricrin

Abstract

Anal. Biochem.

Biochem. Biophys. Res. Commun.

Cell

Cell

J. Invest. Dermatol.

Cell

J. Invest. Dermatol.

J. Ultrastruct. Res.

J. Mol. Biol.

J. Biol. Chem.

J. Biol. Chem.

J. Ultrastruct. Res.

J. Biol. Chem.

J. Biol. Chem.

J. Invest. Dermatol.

J. Invest. Dermatol.

J. Invest. Dermatol.

J. Invest. Dermatol.

J. Invest. Dermatol.

J. Invest. Dermatol.

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Cell

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Differentiation

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Article
Identification of a major keratinocyte cell envelope protein, loricrin