Importance of the nef gene for maintenance of high virus loads and for development of AIDS

doi:10.1016/0092-8674(91)90097-I

Cell

Volume 65, Issue 4, 17 May 1991, Pages 651-662

https://doi.org/10.1016/0092-8674(91)90097-I Get rights and content

Abstract

When rhesus monkeys were infected with a form of cloned SIVmac239 having a premature stop signal at the 93rd codon of nef, revertants with a coding codon at this position quickly and universally came to predominate in the infected animals. This suggests that there are strong selective forces for open functional forms of nef in vivo. Although deletion of nef sequences had no detectable effect on virus replication in cultured cells, deletion of nef sequences dramatically altered the properties of virus in infected rhesus monkeys. Our results indicate that nef is required for maintaining high virus loads during the course of persistent infection in vivo and for full pathologic potential. Thus, nef should become a target for antiviral drug development. Furthermore, the properties of virus with a deletion in nef suggest a means for making live-attenuated strains of virus for experimental vaccine testing.

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Cell

ArticleImportance of the nef gene for maintenance of high virus loads and for development of AIDS

Abstract

Lancet

Virology

J. Mol. Biol.

Meth. Enzymol.

Virology

Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR

Science

A new HTLV-III/LAV encoded antigen detected by antibodies from AIDS patients

Science

Necropsy findings in rhesus monkeys experimentally infected with cultured simian immunodeficiency virus (SIV)/Delta

Vet. Pathol.

Selection of genetic variants of simian immunodeficiency virus in persistently infected rhesus monkeys

J. Virol.

Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses

Nature

Lymphadenopathy in macaques experimentally infected with the simian immunodeficiency virus (SIV)

Am. J. Pathol.

Differential effects of nef on HIV replication: implications for viral pathogenesis in the host

Science

Identification of a protein encoded by the vpu gene of HIV-1

Nature

Identification of HIV-1 vpr product and function

J. Acquir. Immune Defic. Syndr.

Isolation of T-cell tropic HTLV-III-like retrovirus from macaques

Science

Long-term persistent infection of macaque monkeys with the simian immunodeficiency virus

J. Gen. Virol.

Simian immunodeficiency virus from African green monkeys

J. Virol.

The simian immunodeficiency viruses

Annu. Rev. Immunol.

Herpesvirus tamarinus and its relation to herpes simplex virus

J. Gen. Virol.

The use of simian immunodeficiency viruses for AIDS research

Intervirology

Infectious mutants of HTLV-III with changes in the 3′ region and markedly reduced cytopathic effects

Science

The sor gene of HIV-1 is required for efficient virus transmission in vitro

Science

HIV F/3′ orf encodes a phosphorylated GTP-binding protein resembling an oncogene product

Nature

Vpx mutants of HIV-2 are infectious in established cell lines but display a severe defect in peripheral blood lymphocytes

EMBO J.

Nef protein of human immunodeficiency virus type 1: evidence against its role as a transcriptional inhibitor

Article
Importance of the nef gene for maintenance of high virus loads and for development of AIDS