Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death

doi:10.1016/0092-8674(92)90507-9

Cell

Volume 71, Issue 3, 30 October 1992, Pages 369-382

https://doi.org/10.1016/0092-8674(92)90507-9 Get rights and content

Abstract

The Myf-5 gene, a member of the myogenic basic HLH factor family, has been inactivated in mice after homologous recombination in ES cells. Mice lacking Myf-5 were unable to breathe and died immediately after birth, owing to the absence of the major distal part of the ribs. Other skeletal abnormalities, except for complete ossification of the sternum, were not apparent. Histological examination of skeletal muscle from newborn mice revealed no morphological abnormalities. Northern blot analysis demonstrated normal levels of muscle-specific mRNAs including MyoD, myogenin, and Myf-6. However, the appearance of myotomal cells in early somites was delayed by several days. These results suggest that while Myf-5 plays a crucial role in the formation of lateral sclerotome derivatives, Myf-5 is dispensable for the development of skeletal muscle, perhaps because other members of the myogenic HLH family substitute for Myf-5 activity.

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^‡: Present address: McMaster University, Institute for Molecular Biology and Biotechnology, Hamilton, Ontario, Canada, L8S 4K1.

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Cell

ArticleTargeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death

Abstract

J. Biol. Chem.

J. Biol. Chem.

Cell

Cell

Dev. Biol.

Curr. Opin. Cell Biol.

Cell

Cell

Neuromusc. Disorders

Cell

Dev. Biol.

Differentiation

J. Biol. Chem.

Dev. Biol.

Cell

Cell

Curr. Opin. Gen. Dev.

Cell

Myogenic control genes in vertebrates

Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA

Eur. J. Biochem.

Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro

J. Cell Biol.

Theory and Practice of Histological Techniques

The muscle regulatory gene, Myf-6, has a biphasic pattern of expression during early mouse development

J. Cell Biol.

Production and Analysis of Chimeric Mice

The four human muscle regulatory helix-loop-helix proteins Myf-3-Myf-6 exhibit similar hetero-dimerization and DNA binding properties

Nucl. Acids Res.

Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products

EMBO J.

A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T12 fibroblasts

EMBO J.

Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12

EMBO J.

Transcriptional activation domain of the muscle-specific gene-regulatory protein Myf-5

Nature

Baculovirus-expressed myogenic determination factors require E12 complex formation for binding to the myosin-light-chain enhancer

Eur. J. Biochem.

Inhibition of muscle differentiation by the adenovirus E1a protein: repression of the transcriptional activating function of the HLH protein Myf-5

Genes Dev.

Mutagenesis of the myogenin basic region identifies an ancient protein motif critical for activation of myogenesis

Three “myosin adenosine triphosphatase” systems: the nature of their pH lability and sulfhydryl dependence

J. Histochem. Cytochem.

Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos

Genes Dev.

A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program

Genes Dev.

Article
Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death

A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in $10 T 12$ fibroblasts