Paper
Effect of Pluronic F-68 on the mechanical properties of mammalian cells

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Abstract

The mechanical properties of TB/C3 hybridoma cells taken from a continuous culture were measured by micromanipulation. The culture conditions were constant except for the presence or absence of Pluronic F-68 in the medium. It was found that the mean bursting membrane tension and the mean elastic area compressibility modulus of the cells were significantly greater (60% and 120%, respectively) in a medium with 0.05% (w/u) Pluronic F-68 compared to that without Pluronic. Pluronic F-68 therefore affected the strength of the membranes when the cells were exposed to it for a long period of time, i.e., in culture. The short-term effect of Pluronic F-68 on cell strength was also tested by its addition at various levels up to 0.2% (w/v) immediately before the mechanical property measurements. The resulting cell strength depended on the Pluronic concentration, but a significant short-term effect could only be detected above a threshold of 0.1% (w/v). Previous reports on the effect of Pluronic F-68 on animal cell culture are evaluated in the light of these observations.

References (12)

  • E.T. Papoutsakis

    Trends Biotechnol.

    (1991)
  • A. Handa-Corrigan et al.

    Enzyme Microb. Technol.

    (1989)
  • Z. Zhang et al.

    Chem. Eng. Sci.

    (1992)
  • S.K.W. Oh et al.

    J. Biotechnol.

    (1992)
  • J.D. Michaels et al.

    Biotechnol. Bioeng.

    (1991)
  • D.W. Murhammer et al.

    Bio/Technology

    (1988)
There are more references available in the full text version of this article.

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