PaperEffect of Pluronic F-68 on the mechanical properties of mammalian cells
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2021, BiomacromoleculesCHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant
2019, Biochemical Engineering JournalCitation Excerpt :Velez-Suberbie et al. concluded that cells could not survive with direct gas sparging without the addition of shear protectant. Similar conclusions were made by Clincke et al. and Zhang et al. regarding the importance of shear protectants on sustaining cell viability of bioreactors [25,26]. Interestingly, cell viability was maintained at high percentages in mild hypothermic bioreactors operated in similar working volume, stirring speed and aeration rate as the control.
Investigation of interfacial properties of pure and mixed poloxamers for surfactant-mediated shear protection of mammalian cells
2017, Colloids and Surfaces B: BiointerfacesCitation Excerpt :Shear protective additives such as P188 decrease physical damage to suspensions of animal cell cultures by physical and biochemical means [27,28]. Several mechanisms of cell protection by surfactants have been demonstrated, including decreasing cell-to-bubble attachment rate by reducing adsorption of cells to bubbles [4,5,7,29–32], increasing membrane strength through adsorption to the membrane [29,33], and decreasing membrane fluidity [34,35]. The mechanism of shear protection predominantly occurs at the gas-bubble and the membrane interfaces, suggesting that a fundamental understanding of the interactions between poloxamers and gas, liquid, and cell membrane interfaces are important for cell protection.
Effect of F68, F127, and P85 pluronic block copolymers on odontogenic differentiation of human tooth germ stem cells
2013, Journal of EndodonticsCitation Excerpt :Our results revealed that F68 has a great capacity to support odontogenic differentiation of hTGSCs when added into the differentiation medium at 0.05% concentration. This concentration was actually the preferred concentration for F68 in previous studies and our study (18–24). F68-treated cells showed higher expressions of odontogenic markers (ie, COL1A, DSPP, BMP 2, and BMP 7) as well as high ALP activity upon differentiation.
Development of quenching and washing protocols for quantitative intracellular metabolite analysis of uninfected and baculovirus-infected insect cells
2012, MethodsCitation Excerpt :An obvious strategy involves the addition of a cell-protective agent such as Pluronic® F-68 (PF68) to the default NaCl-based QS. PF68 is a non-ionic surfactant that has been used for decades to enable animal cell cultures to be scaled-up in gas-sparged stirred-tank bioreactors [25,49–53], and is typically applied at a concentration of 0.1% v/v in insect cell cultures [54]. In this study, the alternative QS (NaCl + P) was supplemented with twice this level of PF68, which was found to be efficacious in the development of a high cell density fed-batch suspension culture process for HaSNPV biopesticides (personal communication, Leslie Chan).
7.19 - Polymer Nanomechanics
2012, Polymer Science: a Comprehensive Reference: Volume 1-10