Packed-bed column bioreactor for production of enzyme

doi:10.1016/0141-0229(92)90142-B

Enzyme and Microbial Technology

Volume 14, Issue 6, June 1992, Pages 486-488

https://doi.org/10.1016/0141-0229(92)90142-B Get rights and content

Abstract

In solid-state fermentation using a packed-bed column bioreactor for the production of glucoamylase enzyme by Aspergillus niger (RRL isolate), maximum enzyme activity was attained when the column was packed with 0.35g substrate cm⁻³ space loading and allowed to ferment at 30 ± 1°C for 48h with 0.1 l min⁻¹ aeration.

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Temperature, moisture content and concentration of respiration gases have been extensively explored in SSC packed-bed bioreactors. For temperature and moisture content, time-related gradients for the radial and axial coordinates have been published, while for respiration gases concentration the measurements often refers to the whole column (Saucedo-Castañeda et al., 1990; Pandey and Radhakrishnan, 1992; Ghildyal et al., 1994; Zanelato et al., 2012; Casciatori et al., 2016; Perez et al., 2019; Perez et al., 2021). The temperature and gas concentration can be measured online, while the moisture content determination requires the destruction of the sample.
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PaperPacked-bed column bioreactor for production of enzyme

Abstract

Biol. Wastes

J. Ferment. Technol.

Biol. Wastes

Biores. Technol.

Biotechnol. Bioeng.

J. Basic Microbiol.

Biotechnol. Lett.

Biotechnol. Lett.

J. Ferment. Technol.

Paper
Packed-bed column bioreactor for production of enzyme