Regular articleA common plasmid of Chlamydia trachomatis
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Development of a duplex recombinase-aided amplification assay for direct detection of Mycoplasma pneumoniae and Chlamydia trachomatis in clinical samples
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2020, Indian Journal of Medical MicrobiologyA simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs
2017, Diagnostic Microbiology and Infectious DiseaseA multiplex PCR assay for the simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis
2015, Experimental and Molecular PathologyCitation Excerpt :In our primer design, we targeted multi-copy regions within the three STI genomes to achieve highest sensitivity. For Chlamydia trachomatis, we targeted the multi-copy cryptic plasmid thus, not only enhancing sensitivity, but also ensuring the detection of all Chlamydia trachomatis serovars including the Swedish nvCT serovar (Caliendo et al., 2005; Palmer and Falkow, 1986; Unemo & Clarke, 2011). After extensive PCR optimization, we devised a touchdown PCR protocol with extension temperatures gradually decreasing from 70 °C to 60 °C as shown in Fig. 1A. With this optimal protocol, we were able to precisely resolve the melt peaks for TV at 78 °C, NG at 82.3 °C, CT at 85.7 °C, and IC2M at 92 °C (Fig. 1B).
Dendrimer-enabled transformation of Chlamydia trachomatis
2013, Microbial PathogenesisCitation Excerpt :Importantly, studies from our group have indicated that many of these latter genes are involved in/required for persistent infection [e.g., [18]; APH personal communication]. Virtually all chlamydial strains also harbor a plasmid of ∼7.5 kbp [19–22], but studies have demonstrated that the functions encoded on it are not required for survival [e.g., [23–26]; see also [27]]. Interestingly, C. trachomatis produces iodine-stainable glycogen in its inclusions during the developmental cycle [e.g., [24]].
Assessment of the load and transcriptional dynamics of Chlamydia trachomatis plasmid according to strains' tissue tropism
2013, Microbiological ResearchCitation Excerpt :Apart from the highly conserved chromosome (>98% of similarity among strains) (Harris et al., 2012), C. trachomatis naturally harbours a ∼7.5 kb plasmid, which was firstly identified in 1980 (Lovett et al., 1980). This plasmid is also highly conserved among strains (Comanducci et al., 1990; Harris et al., 2012) and possesses eight open reading frames (ORFs) that are known to be transcribed and translated (Palmer and Falkow, 1986; Ricci et al., 1993, 1995), as well as two small anti-sense RNAs (sRNAs) (Ricci et al., 1993; Albrecht et al., 2010). The presence of the highly conserved plasmid among several chlamydial species suggests that it was acquired early in the evolution of Chlamydiae and must be subjected to a strong selective pressure for its maintenance by these bacteria that shows a reduced chromosome size, given their intracellular nature (Andersson and Kurland, 1998; Zomorodipour and Andersson, 1999).