Characterization of a plasmid isolated from Branhamella catarrhalis and detection of plasmid sequences within the genome of a B. catarrhalis strain

doi:10.1016/0147-619X(88)90020-0

Plasmid

Volume 20, Issue 2, September 1988, Pages 158-162

https://doi.org/10.1016/0147-619X(88)90020-0 Get rights and content

Abstract

We isolated a 12.2-kb plasmid from two clinical strains of Branhamella catarrhalis and evaluated its distribution among other B. catarrhalis strains by colony hybridization experiments using the whole plasmid as a probe. Homology was detected with the two plasmid-bearing strains and also with a third B. catarrhalis strain named E7, which is plasmidless. Southern transfer analysis of total digested E7 DNA using the purified plasmid as a probe revealed a single band of hybridization, different from those observed in plasmid-bearing strains, for each restriction enzyme used. The region of the plasmid hybridizing with DNA of strain E7 was located within a 4.5-kb SstI-EcoRV fragment. No homology was noted between our B. catarrhalis plasmid and about 20 other strains of different genera tested by colony hybridization.

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Cited by (18)

Development of a LacZ-based transcriptional reporter system for use with Moraxella catarrhalis
2013, Plasmid
The lack of a transcriptional reporter system for use in Moraxella catarrhalis has hindered studies of gene regulation in this pathogen. PCR and recombinant DNA methods were used to insert a multicloning site (MCS) and promoterless full-length Escherichia coli lacZ gene, flanked by transcriptional terminators both immediately upstream and downstream, into the M. catarrhalis recombinant plasmid pWW115. Insertion into the MCS in the newly constructed plasmid pASE222 of M. catarrhalis promoter regions controlled by either a repressor (i.e., NsrR) or activator (i.e., PhoB) yielded transcriptional fusion constructs that were appropriately responsive to signal inputs dependent on the host strain genotype, as measured quantitatively by means of a Miller β-galactosidase assay. The transcriptional reporter plasmid pASE222 should prove to be a useful tool for rapid screening of factors affecting gene expression in M. catarrhalis.
Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis
2006, Plasmid
The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catarrhalis uspA2 mutant.
Development of a shuttle vector for Moraxella catarrhalis
2006, Plasmid
Efforts to perform genetic analysis in Moraxella catarrhalis have been hampered by the lack of a cloning vector. M. catarrhalis strain E22 was previously shown to contain plasmid pLQ510 which lacked a selectable antibiotic resistance marker. Several methods were used to eliminate unnecessary DNA from pLQ510. Then, a 1.2 kb spectinomycin resistance cartridge, a multiple cloning site, and the origin of replication from pACYC184 were cloned into this plasmid backbone to obtain the 7.2 kb plasmid pWW102B. This new plasmid could replicate in M. catarrhalis as well as in both Escherichia coli and Haemophilus influenzae. This shuttle vector was used to clone and express two different M. catarrhalis genes, respectively, encoding an adhesin and a protein involved in serum resistance. When these two plasmids were introduced into appropriate M. catarrhalis mutants, they complemented the phenotypic deficiency of each mutant. This is the first report of functional complementation in trans in this pathogen.
A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis possibly useful as a cloning and expression vector within this species
2005, Plasmid
A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJH04) allowed selection and isolation of the plasmid in Escherichia coli. Natural transformation of pEMCJH04 into M. catarrhalis was successful for 25% (3/12) of non-isogenic isolates, with no link between (lack of) transformability and genetic lineage or (lack of) transformability and complement phenotype, though the transformation efficiency was found to be rather low at approximately 615 CFU/μg (range = 60–1040 CFU/μg ). This is only the second publication detailing a plasmid isolated from this important respiratory pathogen, and the ability to clone and express foreign proteins in M. catarrhalis using pEMCJH04 could help in the development of a vaccine expression vector, as well as providing a useful tool for studying promoter activity and in complementation studies of gene knockout mutants.
Structural analysis of plasmid pLQ510 from Moraxella catarrhalis E22
1999, Plasmid
The complete nucleotide sequence of plasmid pLQ510 from Moraxella catarrhalis strain E22 has been determined. This plasmid contained 12,082 bp with 38% GC content. Five open reading frames that encoded predicted proteins with homology to plasmid-encoded proteins from other bacteria were identified. A putative origin of replication that contained an AT-rich region followed by four direct repeats and an inverted repeat was identified.
Moraxella (Branhamella) catarrhalis: Restriction enzyme analysis typing with HinfI, HaeIII and PstI
1995, FEMS Immunology and Medical Microbiology

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¹: Present address: Molecular Biology Department, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

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Plasmid

Abstract

References (24)

Integration of the plasmid prophages P1and P7 into the chromosome of Escherichia coli

J. Mol. Biol

Branhamella (Neisseria) catarrhalis as a pathogen

Lancet

Pathogenicity of β-lactamase production by Branhamella (Neisseria) catarrhalis

Lancet

Comparative efficacies of erythromycin-sulfisoxasole and cefaclor in acute otitis media: A double blind randomized trial

Pediatr. Infect. Dis. J

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucleic Acids Res

Novel β-lactamase from Branhamella catarrhalis

Ann. Microbiol. Institut Pasteur B

Branhamella (Neisseria) catarrhalis: Criteria for Laboratory identification

J. Clin. Microbiol

Antibiotic susceptibility of β-lactamase producing strains of Branhamella (Neisseria) catarrhalis

Antimicrob. Agents Chemother

Characterization of the plasmid-mediated β-lactamase in Branhamella catarrhalis with special reference to substrate affinity

J. Antimicrob. Chemother

Intragenic variation by site specific recombination in the cryptic plasmid of Neisseria gonorrhoeae

J. Bacteriol

Moraxella, Neisseria, Branhamella and Acinetobacter

Annu. Rev. Microbiol

Transfer of β-lactamase production in Branhamella catarrhalis

Scand. J. Infect. Dis

Cited by (18)

Development of a LacZ-based transcriptional reporter system for use with Moraxella catarrhalis

Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis

Development of a shuttle vector for Moraxella catarrhalis

A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis possibly useful as a cloning and expression vector within this species

Structural analysis of plasmid pLQ510 from Moraxella catarrhalis E22

Moraxella (Branhamella) catarrhalis: Restriction enzyme analysis typing with HinfI, HaeIII and PstI

Short communicationCharacterization of a plasmid isolated from Branhamella catarrhalis and detection of plasmid sequences within the genome of a B. catarrhalis strain

Abstract

J. Mol. Biol

Lancet

Lancet

Comparative efficacies of erythromycin-sulfisoxasole and cefaclor in acute otitis media: A double blind randomized trial

Pediatr. Infect. Dis. J

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucleic Acids Res

Novel β-lactamase from Branhamella catarrhalis

Ann. Microbiol. Institut Pasteur B

Branhamella (Neisseria) catarrhalis: Criteria for Laboratory identification

J. Clin. Microbiol

Antibiotic susceptibility of β-lactamase producing strains of Branhamella (Neisseria) catarrhalis

Antimicrob. Agents Chemother

Characterization of the plasmid-mediated β-lactamase in Branhamella catarrhalis with special reference to substrate affinity

J. Antimicrob. Chemother

Intragenic variation by site specific recombination in the cryptic plasmid of Neisseria gonorrhoeae

J. Bacteriol

Moraxella, Neisseria, Branhamella and Acinetobacter

Annu. Rev. Microbiol

Transfer of β-lactamase production in Branhamella catarrhalis

Scand. J. Infect. Dis

Short communication
Characterization of a plasmid isolated from Branhamella catarrhalis and detection of plasmid sequences within the genome of a B. catarrhalis strain