Characterization of antigen-expressing Plasmodium falciparum cDNA clones that are reactive with parasite inhibitory antibodies
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Cited by (37)
Plasmodium falciparum serine repeat antigen 5 (SE36) as a malaria vaccine candidate
2011, VaccineCitation Excerpt :Originally described under various names, including Pf140 [21], p113 [22], p126 [23], or SERP [24], serine repeat antigen 5 (SERA5) was first identified as an abundant, exported, soluble late-trophozoite and schizont stage protein of P. falciparum that accumulates in the parasitophorous vacuole, is released in soluble form at schizont rupture, and could induce antibodies that either protected against blood stage infection in vivo [21] or inhibited parasite growth in vitro [22]. Cloning of the first SERA gene revealed the presence of an extraordinarily serine rich amino terminal domain, on which the protein was renamed [25–27]. The primary sequence exhibits a secretory signal sequence with no membrane anchor domain, consistent with the highly soluble nature of the protein.
Plasmodium falciparum serine-repeat antigen (SERA) forms a homodimer through disulfide bond
2005, Parasitology InternationalA gene-family encoding small exported proteins is conserved across Plasmodium genus
2003, Molecular and Biochemical ParasitologySerine repeat antigen (SERA5) is predominantly expressed among the SERA multigene family of Plasmodium falciparum, and the acquired antibody titers correlate with serum inhibition of the parasite growth
2002, Journal of Biological ChemistryCitation Excerpt :Transcriptional profile among the SERA multigene family measured by RT-PCR with limited PCR cycles (Fig. 2), the Northern blot analysis (Fig. 3), and the real time quantitative PCR (Fig. 4) consistently showed that the abundance of SERA5 RNA was always higher than those of other SERA genes and was in 3–5-fold excess of MSP-1 RNA. This observation is in good accord with the previous report that SERA5 cDNA copies were abundant, since 0.5–1.5% of clones are SERA cDNAs in the cDNA library constructed from poly(A) RNA of erythrocytic stage P. falciparum parasites (38). Almost all of the SERA multigene family genes examined here were most actively transcribed in trophozoite and schizont stages (Fig. 5).
Three multigene families in Plasmodium parasites: Facts and questions
2002, International Journal for Parasitology
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Present address: Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan.