Regular paperStage-regulated expression of cruzipain, the major cysteine protease of Trypanosoma cruzi is independent of the level of RNA☆
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The Trypanosoma cruzi genome; conserved core genes and extremely variable surface molecule families
2011, Research in MicrobiologyCitation Excerpt :It is clear that the repetitive nature of many housekeeping genes is conserved between strains and that there are mechanisms to maintain sequence similarity between repeat copies within tandem arrays. The latter phenomenon is found, for example, for cruzipain, a cysteine protease, that is present as two arrays of more than 20 1.8 kb copies on a homologous pair of chromosomes (Tomas and Kelly, 1996; Arner et al., 2007). In the CL Brener strain, the gene copies within each homologous array are close to identical, while there is 3% polymorphism between the homologs (observed in our laboratory by clone sequencing).
Aspects of Trypanosoma cruzi Stage Differentiation
2011, Advances in ParasitologyCitation Excerpt :The cruzipain genes are transcribed as a 1.8-kb transcript which is constitutively expressed throughout the parasite life cycle. Interestingly, the protein abundance and consequently the enzymatic activity are four to five times higher in the insect epimastigote stage than in the trypomastigote and amastigote stages (Hanke et al., 1996). Accordingly, it has been suggested that the developmental regulation of cruzipain expression is likely to be mostly translational and/or post-translational (Tomas and Kelly, 1996).
Proteomic analysis of two Trypanosoma cruzi zymodeme 3 strains
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Kinetoplastid papain-like cysteine peptidases
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Nucleotide sequence data reported in this paper are available in the GenBank database under the accession numbers U41444, U41454 and U41511.