Age-dependence of free radical-induced oxidative damage in ischemic-reperfused rat heart

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Abstract

Oxygen free radical-induced oxidative damage is involved in both aging and ischemia-reperfusion. The purpose of this study was to determine the aging-induced oxidative alterations in rat heart as well as the age-dependence of heart injury following ischemia-reperfusion. A comparative study was performed on young and old ischemic-reperfused rat hearts. Protein oxidation and the ascorbyl radical level in heart tissue were determined in order to characterize the oxidative stress. Comparing the control conditions, old hearts have 31% more oxidized proteins as measured by protein carbonyl content, and 18% lower ascorbyl radical level as determined by ESR, than young ones. The extent of increase of protein oxidation and ascorbyl free radical depletion induced by ischemia-reperfusion is less pronounced in the old hearts (7 and 8% respectively), as compared to the young ones (55 and 21% respectively). Pre-treatment with a free radical scavenger, such as centrophenoxine, diminished the ischemia-reperfusion injury in both young and old rat hearts.

References (31)

  • D. Roy et al.

    Effect of centrophenoxine on the antioxidative enzymes in various regions of the aging rat brain

    Exp. Geront.

    (1983)
  • P.E. Spoerri et al.

    Accumulation of lipofuscin in the myocardium of senile guinea pigs: dissolution and removal of lipofuscin following dimethylaminoethyl-p-chlorophenoxy-acetate administration: an electron microscope study

    Mech. Ageing Dev.

    (1974)
  • P.E. Starke-Reed et al.

    Protein oxidation and proteolysis during aging and oxidative stress

    Arch. Biochem. Biophys.

    (1989)
  • B. Tavazzi et al.

    Malondialdehyde production and ascorbate decrease are associated to the reperfusion of the isolated post-ischemic rat heart

    Free Radic. Biol. Med.

    (1992)
  • A. Tosaki et al.

    Effects of SOD, catalase, and a novel antiarrhytmic drug, EGB 761, on reperfusion-induced arrhythmias in isolated rat hearts

    Free Radic. Biol. Med.

    (1993)

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