The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells

doi:10.1016/0168-1656(89)90128-4

Journal of Biotechnology

Volume 12, Issue 1, October 1989, Pages 45-61

https://doi.org/10.1016/0168-1656(89)90128-4 Get rights and content

Abstract

The response of 3 murine hybridomas to increasing speeds of agitation from 100 to 450 rpm with propellers and Rushton turbines in surface-aerated bioreactors of 1.4 l has been determined with pO₂ being kept above a minimum level of 20%. Cell growth and viability, antibody production, glucose consumption, lactate production and metabolic activity have been measured and found to be unaffected over this range of speeds. Scanning electron micrographs (SEM) showed no detectable cell damage even at the highest intensity of agitation. However, once the culture was continuously sparged with air the net cell growth rate and the maximum cell number fell markedly, that fall increasing with increasing agitator speed. To generalise these findings and compare them with literature reports, the implications of this speed range on the turbulence parameters have been discussed. The implications for scale-up are also considered.

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Research articleThe effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells

Abstract

Chem. Eng. Sci.

Chem. Eng. Sci.

Chem. Eng. Sci.

Chem. Eng. Sci.

J. Immunol. Methods

J. Biotechnol.

Chem. Eng. Sci.

Chem. Eng. Sci.

J. Fluid Mech.

Use of the MTT Assay for the Study of Hybridoma Cells in Homogeneous and Heterogeneous Cultures

Flow Cytometric Studies during Synchronous and Asynchronous Suspension Cultures of Hybridoma Cells

Effect of shear on the death of two strains of mammalian tissue cells

Biotechnol. Bioeng.

Shear and Mixing in Large-Scale Suspension Culture

Large-scale production of monoclonal antibodies in suspension culture

Biotechnol. Bioeng.

Enzyme inactivation with shearing

Biotechnol. Bioeng.

Hydrodynamic effect on cells in agitated tissue culture reactor

Bioproc. Eng.

Hydrodynamic effects on animal cells grown in microcarrier cultures

Biotechnol. Bioeng.

Effects of microcarrier concentration in animal cell culture

Biotechnol. Bioeng.

Viscous reduction of turbulent damage in animal cell culture

Biotechnol. Bioeng.

Flow and turbulence in a stirred tank

AIChE J.

Turbulence Phenomena

Research article
The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells